Product Pathways - NF-kB Signaling
Phospho-IκBα (Ser32/36) (5A5) Mouse mAb (Sepharose Bead Conjugate) #4088
|4088S||400 µl (40 immunoprecipitations)||---||In Stock||---|
|4088||carrier free and custom formulation / quantity||email request|
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|IP||1:20||Human, Mouse, Rat, Monkey, Dog||Endogenous||40||Mouse IgG1|
Species cross-reactivity is determined by western blot using the unconjugated antibody.
Applications Key: IP=Immunoprecipitation
Species predicted to react based on 100% sequence homology: Bovine, Pig.
Specificity / Sensitivity
Phospho-IκBα (Ser32/36) (5A5) Mouse mAb (Sepharose Bead Conjugate) detects endogenous levels of IκBα only when phosphorylated at Ser32/36.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser32/36 of human IκBα.
Immunoprecipitation of NIH/3T3 cell lysates, untreated or TNF-α-treated, using Mouse IgG (Sepharose Bead Conjugate) #3420 (Lanes 1 and 2) and Immobilized Phospho-IκBα (Ser32/36) (5A5) Mouse mAb (Bead Conjugate) (Lanes 3 and 4). The blot was probed using Phospho-IκBα (Ser32/36) (5A5) Mouse mAb #9246.
This Cell Signaling Technology antibody is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated sepharose beads. Phospho-IκBα (Ser32/36) (5A5) Mouse mAb (Sepharose Bead Conjugate) is useful for the immunoprecipitation of phosphorylated IκBα.
The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins (1-3). Activation occurs via phosphorylation of IκBα at Ser32 and Ser36 followed by proteasome-mediated degradation that results in the release and nuclear translocation of active NF-κB (3-7). IκBα phosphorylation and resulting Rel-dependent transcription are activated by a highly diverse group of extracellular signals including inflammatory cytokines, growth factors, and chemokines. Kinases that phosphorylate IκB at these activating sites have been identified (8).
- Baeuerle, P.A. and Baltimore, D. (1988) Science 242, 540-6.
- Beg, A.A. and Baldwin, A.S. (1993) Genes Dev 7, 2064-70.
- Finco, T.S. et al. (1994) Proc Natl Acad Sci USA 91, 11884-8.
- Brown, K. et al. (1995) Science 267, 1485-8.
- Brockman, J.A. et al. (1995) Mol Cell Biol 15, 2809-18.
- Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83.
- Chen, Z.J. et al. (1996) Cell 84, 853-62.
- Karin, M. and Ben-Neriah, Y. (2000) Annu Rev Immunol 18, 621-63.
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- 3420 Mouse IgG (Sepharose Bead Conjugate)
- 9936 NF-κB Pathway Sampler Kit
- 8219 PhosphoPlus® IκBα (Ser32/Ser36) Antibody Duet
- 8214 PhosphoPlus® NF-κB p65/RelA (Ser536) Antibody Duet
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.