Cell Signaling Technology
XP Monoclonal Antibody

Product Pathways - Jak/Stat Pathway

Jak2 (D2E12) XP® Rabbit mAb (Sepharose Bead Conjugate) #4089

Applications Reactivity Sensitivity MW (kDa) Isotype
IP H M R (Mk) Endogenous 125 Rabbit IgG

Applications Key:  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Jak2 (D2E12) XP® Rabbit mAb (Sepharose Bead Conjugate) detects endogenous levels of total Jak2 protein. No cross-reactivity was observed with other family members.

Source / Purification

Monoclonal antibody is produced by immunzing animals with a synthetic peptide corresponding to residues surrounding Pro841 of Jak2.

IP

IP

Immunoprecipitation of extracts from K562 cells, using Jak2 (D2E12) XP® Rabbit mAb (Sepharose Bead Conjugate) (lane 2). XP® Rabbit IgG (DA1E) mAb Isotype Control (Sepharose Bead Conjugate) #3423 was used as a negative control (lane 1). The western blot was probed using Jak2 (D2E12) XP® Rabbit mAb #3230 and detected with Mouse Anti-Rabbit IgG (Conformation Specific) (L27A9) mAb #3678 followed by Anti-mouse IgG, HRP-linked Antibody #7076.

Description

This Cell Signaling Technology antibody is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated sepharose beads. Jak2 (D2E12) XP® Rabbit mAb (Sepharose Bead Conjugate) is useful for the immunoprecipitation of Jak2 proteins.

Background

Members of the Janus family of tyrosine kinases (Jak1, Jak2, Jak3, and Tyk2) are activated by ligands binding to a number of associated cytokine receptors (1). Upon cytokine receptor activation, Jak proteins become autophosphorylated and phosphorylate their associated receptors to provide multiple binding sites for signaling proteins. These associated signaling proteins, such as Stats (2), Shc (3), insulin receptor substrates (4), and focal adhesion kinase (FAK) (5), typically contain SH2 or other phospho-tyrosine-binding domains.

  1. Leonard, W.J. and O'Shea, J.J. (1998) Annu Rev Immunol 16, 293-322.
  2. Darnell, J.E. (1997) Science 277, 1630-5.
  3. VanderKuur, J. et al. (1995) J Biol Chem 270, 7587-93.
  4. Argetsinger, L.S. et al. (1995) J Biol Chem 270, 14685-92.
  5. Zhu, T. et al. (1998) J Biol Chem 273, 10682-9.

Application References

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Companion Products

Jak antibodies produced under license (granting certain rights including those under U. S. Patent No. 5,658,791) from Chemicon International, Inc.

For Research Use Only. Not For Use In Diagnostic Procedures.

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