Cell Signaling Technology

Product Pathways - MAPK Signaling

Phospho-p38 MAPK (Thr180/Tyr182) (3D7) Rabbit mAb (Biotinylated) #4092

Applications Reactivity Sensitivity MW (kDa) Isotype
W F H M R Mk Pg Sc Dm Endogenous 43 Rabbit IgG

Applications Key:  W=Western Blotting  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  Pg=Pig  Sc=S. cerevisiae  Dm=D. melanogaster
Species cross-reactivity is determined by Western blot.

Protocols

Specificity / Sensitivity

Phospho-p38 MAP Kinase (Thr180/Tyr182) (3D7) Rabbit mAb (Biotinylated) detects endogenous levels of p38 MAPK only when phosphorylated at both Thr180 and Tyr182. This antibody does not cross-react with the phosphorylated forms of either p42/44 MAPK or SAPK/JNK.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr180/Tyr182 of human p38 MAPK.

Western Blotting

Western Blotting

Western blot analysis of p38 MAPK Control Cell Extracts #9213, untreated or anisomycin-treated, using Phospho-p38 MAPK (Thr180/Tyr182) (3D7) Rabbit mAb (Biotinylated).

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells, untreated (blue) or anisomcyin-treated (green), using Phospho-p38 MAPK (Thr180/Tyr182) (3D7) Rabbit mAb (Biotinylated).

Description

This Cell Signaling Technology (CST) antibody is conjugated to biotin under optimal conditions. The unconjugated Phospho-p38 MAPK (Thr180/Tyr182) (3D7) Rabbit mAb #9215 reacts with human, mouse, rat, monkey, pig, S. cerevisiae, and D. melanogaster phospho-p38 MAP kinase (Thr180/Tyr182). CST expects that Phospho-p38 MAP Kinase (Thr180/Tyr182) (3D7) Rabbit mAb (Biotinylated) will also recognize phospho-p38 MAP kinase (Thr180/Tyr182) in these species.

Background

p38 MAP kinase (MAPK), also called RK (1) or CSBP (2), is the mammalian orthologue of the yeast HOG kinase that participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAP kinase, p38α, β, γ (also known as ERK6 or SAPK3) and δ (also known as SAPK4) have been identified. Similar to the SAPK/JNK pathway, p38 MAP kinase is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharides (LPS), UV light and growth factors (1-5). MKK3, MKK6 and SEK activate p38 MAP kinase by phosphorylation at Thr180 and Tyr182. Activated p38 MAP kinase has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6) and MEF2 (5-8).

  1. Rouse, J. et al. (1994) Cell 78, 1027-1037.
  2. Han, J. et al. (1994) Science 265, 808-811.
  3. Lee, J.C. et al. (1994) Nature 372, 739-746.
  4. Freshney, N.W. et al. (1994) Cell 78, 1039-1049.
  5. Raingeaud, J. et al. (1995) J. Biol. Chem. 270, 7420-7426.
  6. Zervos, A.S. et al. (1995) Proc. Natl. Acad. Sci. USA 92, 10531-10534.
  7. Zhao, M. et al. (1999) Mol. Cell. Biol. 19, 21-30.
  8. Yang, S.H. et al. (1999) Mol. Cell. Biol. 19, 4028-4038.

Application References

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Companion Products

Rabbit monoclonal antibody is produced under license (granting certain rights including those under U. S. Patent No. 5,675,063 and U.S.S.N. 11/476,277) from Epitomics, Inc.

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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