Product Pathways - Companion Products
Rabbit (DA1E) mAb IgG XP® Isotype Control (Biotinylated) #4096
|IP F||Endogenous||Rabbit IgG|
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
Rabbit (DA1E) mAb IgG XP® Isotype Control (Biotinylated) is not directed against any known antigen. It functions as an isotype control for rabbit IgG monoclonal antibodies.
Source / Purification
Immunoprecipitation of extracts from NIH/3T3 cells treated with PDGF using Rabbit (DA1E) mAb IgG XP® Isotype Control (Biotinylated) (lane 1) and Phospho-Akt (Ser473) (193H12) Rabbit mAb (Biotinylated) #3637 (lane 2). Biotinylated antibodies were pulled down using Streptavidin (Bead Conjugate) #3419. Western blot analysis was performed using Phospho-Akt (Ser473) (587F11) Mouse mAb #4051.
Rabbit (DA1E) mAb IgG XP® Isotype Control (Biotinylated) antibody was conjugated to biotin under optimal condtions and tested in-house for direct flow cytometry and western blot.
Isotype control antibodies are used to estimate the nonspecific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.
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- 3900 Rabbit (DA1E) mAb IgG XP® Isotype Control
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- 2985 Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 647 Conjugate)
For Research Use Only. Not For Use In Diagnostic Procedures.