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Mouse (MOPC-21) mAb IgG1 Isotype Control (Biotinylated) #4097
| Applications | Reactivity | MW (kDa) | Isotype |
|---|---|---|---|
| IP F | N/A | Mouse IgG1 |
Applications Key:
IP=Immunoprecipitation
F=Flow Cytometry
Reactivity Key:
Species cross-reactivity is determined by Western blot.
Protocols
- 4097:
- Flow, Immunoprecipitation
Specificity / Sensitivity
Mouse (MOPC-21) mAb IgG1 Isotype Control (Biotinylated) has unknown specificity and was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed rat and human tissues.
Source / Purification
Mouse (MOPC-21) mAb IgG1 Isotype Control (Biotinylated) was purified by affinity chromatography.
IP
Immunoprecipitation of extracts from Jurkat cells treated with either LY294002 #9901 (1) or Calyculin A #9902 (2), using Akt (pan) (40D4) Mouse mAb (Biotinylated) #4821 and Mouse (MOPC-21) mAb IgG1 Isotype Control (Biotinylated). Immunocomplexes were pulled down using Immobilized Streptavidin (Bead Conjugate) #3419. Western blotting analysis was performed using Akt (pan) (C67E7) Rabbit mAb #4691.
IP
Immunoprecipitation of extracts from Jurkat cells treated with either LY294002 #9901 (1) or Calyculin A #9902 (2), using Phospho-Akt (Ser473) (587F11) Mouse mAb (Biotinylated) #5102 and Mouse (MOPC-21) mAb IgG1 Isotype Control (Biotinylated). Immunocomplexes were pulled down using Immobilized Streptavidin (Bead Conjugate) #3419. Western blotting analysis was performed using Phospho-Akt (Ser473) (D9E) Rabbit mAb #4060.
Flow Cytometry
Flow cytometric analysis of Jurkat cells using Akt (pan) (40D4) Mouse mAb (Biotinylated) #4821 (blue) compared to Mouse (MOPC-21) mAb IgG1 Isotype Control (Biotinylated) (red).
Description
This Cell Signaling Technology monoclonal antibody is conjugated to biotin under optimal conditions.
Background
Isotype control antibodies are used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.
Application References
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Companion Products
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
