Cell Signaling Technology

Product Pathways - Metabolism

SNARK/NUAK2 Antibody #4100

Applications Reactivity Sensitivity MW (kDa) Source
W M (H) (R) Endogenous 70 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

SNARK/NUAK2 Antibody detects endogenous levels of total SNARK/NUAK2 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human SNARK/NUAK2. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from the mouse tibialis anterior (TA) muscle using SNARK/NUAK2 Antibody.

Background

SNARK was identified as an SNF1/AMPK-related kinase and member of the AMPK catalytic subunit family (1,2). This enzyme was separately identified as a TNFα-induced SNF1-like kinase 2 (NUAK2) (3). The kinase activity of SNARK/NUAK2 is increased by AMP and AICAR (1). SNARK/NUAK2 activity is regulated by a variety of cellular stresses such as endoplasmic reticulum (ER) stress and oxidative stresses (4), suggesting that SNARK/NUAK2 is a signaling molecule involved in the cell stress response (4).

  1. Lefebvre, D.L. et al. (2001) Biochem J 355, 297-305.
  2. Suzuki, A. et al. (2003) Biochem Biophys Res Commun 311, 156-61.
  3. Yamamoto, H. et al. (2008) Biochem Biophys Res Commun 365, 541-7.
  4. Lefebvre, D.L. and Rosen, C.F. (2005) Biochim Biophys Acta 1724, 71-85.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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