Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

ROR1 Antibody #4102

Applications Reactivity Sensitivity MW (kDa) Source
W M (H) Transfected Only 135 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

ROR1 Antibody detects transfected levels of ROR1 proteins. It does not cross-react with ROR2.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the residues at the amino-terminal sequence of human ROR1. The antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HEK293 cells, untransfected or transfected with HA-tagged mROR1, using ROR1 Antibody (upper) or HA-tag Antibody #2362 (lower).

Background

ROR1 and ROR2 are orphan receptor tyrosine kinases that are most closely related to MuSK and the Trk family of neurotrophin receptors. They are characterized by the presence of extracellular frizzled-like cysteine-rich domains and membrane-proximal kringle domains, both of which are assumed to mediate protein-protein interactions (1-3). The ROR family RTKs are evolutionarily conserved among Caenorhabditis elegans, Drosophila, mice, and humans (1,4). Although the functions of ROR kinases are unknown, similarities between ROR and MuSK and Trk kinases have led to speculation that ROR kinases regulate synaptic development. CAM-1, a C. elegans ortholog of the ROR family RTKs, plays several important roles in regulating cellular migration, polarity of asymmetric cell divisions, and axonal outgrowth of neurons during nematode development (4). mROR1 and mROR2 may play differential roles during the development of the nervous system (5).

  1. Al-Shawi , R. et al. (2001) Dev .Genes Evol. 211, 161-171.
  2. Nomi, M. et al. (2001) Mol. Cell. Biol. 21, 8329-8235.
  3. Roszmusz, E. et al. (2001) J. Biol. Chem. 276, 18485-18490.
  4. Forrester, . et al. (1999) Nature 400, 881-885.
  5. Oishi, I. (1999) Genes Cells 4, 41-56.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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