Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

Ku70 (V540) Antibody #4104

Applications Reactivity Sensitivity MW (kDa) Source
W H Mk (Hm) Endogenous 70 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  Hm=Hamster  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Ku70 (V540) Antibody detects endogenous levels of total Ku70 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of the human Ku70 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa and COS cells using Ku70 (V540) Antibody.

Background

Ku is a heterodimeric protein composed of two subunits (Ku70 and Ku80) originally identified by researchers as autoantigens associated with several autoimmune diseases including scleroderma, polymyositis, and systemic lupus erythematosus (1). Ku is an abundant, ubiquitously expressed nuclear protein that binds to and stabilizes the ends of DNA at telomeres or double-stranded DNA breaks (2-5). The Ku70/Ku80 heterodimer has ATP-dependent DNA helicase activity and functions as the DNA-binding regulatory component of DNA-dependent protein kinase (DNA-PK) (6-8). The assembly of the DNA-PK complex at DNA ends is required for nonhomologous end-joining (NHEJ), one mechanism involved in double-stranded DNA break repair and V(D)J recombination (8). DNA-PK has been shown to phosphorylate many proteins, including p53, serum response factor, c-Jun, c-Fos, c-Myc, Oct-1, Sp-1, and RNA polymerase II (1,8). The combined activities of Ku70/Ku80 and DNA-PK implicate Ku in many cellular functions, including cell cycle regulation, DNA replication and repair, telomere maintenance, recombination, and transcriptional activation.

  1. Tuteja, R. and Tuteja, N. (2000) Crit. Rev. Biochem. Mol. Biol. 35, 1-33.
  2. Blier, P.R. et al. (1993) J. Biol. Chem. 268, 7594-7601.
  3. Jin, S. and Weaver, D.T. (1997) EMBO J. 16, 6874-6885.
  4. Boulton, S.J. and Jackson, S.P. (1998) EMBO J. 17, 1819-1828.
  5. Gravel, S. et al. (1998) Science 280, 741-744.
  6. Cao, Q.P. et al. (1994) Biochemistry 33, 8548-8557.
  7. Lees-Miller, S.P. et al. (1990) Mol. Cell Biol. 10, 6472-6481.
  8. Collis, S.J. et al. (2005) Oncogene 24, 949-961.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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