Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

ROR2 Antibody #4105

Applications Reactivity Sensitivity MW (kDa) Source
W IP M (H) Transfected Only 135 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

ROR2 Antibody detects transfected levels of ROR2 proteins. It does not cross-react with ROR1.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino-terminal sequence of human ROR2. Antibodies are purified by protein A and peptide-affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HEK293 cells, untransfected or transfected with DYKDDDDK-tagged mouse ROR2, using ROR2 Antibody (upper) or DYKDDDDK Tag Antibody (Binds to same epitope as Sigma's Anti-FLAG® M2 Antibody) #2368 (lower).

Background

ROR1 and ROR2 are orphan receptor tyrosine kinases that are most closely related to MuSK and the Trk family of neurotrophin receptors. They are characterized by the presence of extracellular frizzled-like cysteine-rich domains and membrane-proximal kringle domains, both of which are assumed to mediate protein-protein interactions (1-3). The ROR family RTKs are evolutionarily conserved among Caenorhabditis elegans, Drosophila, mice, and humans (1,4). Although the functions of ROR kinases are unknown, similarities between ROR and MuSK and Trk kinases have led to speculation that ROR kinases regulate synaptic development. CAM-1, a C. elegans ortholog of the ROR family RTKs, plays several important roles in regulating cellular migration, polarity of asymmetric cell divisions, and axonal outgrowth of neurons during nematode development (4). mROR1 and mROR2 may play differential roles during the development of the nervous system (5).

  1. Al-Shawi , R. et al. (2001) Dev .Genes Evol. 211, 161-171.
  2. Nomi, M. et al. (2001) Mol. Cell. Biol. 21, 8329-8235.
  3. Roszmusz, E. et al. (2001) J. Biol. Chem. 276, 18485-18490.
  4. Forrester, . et al. (1999) Nature 400, 881-885.
  5. Oishi, I. (1999) Genes Cells 4, 41-56.

Application References

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Anti-FLAG® is a registered trademark of Sigma-Aldrich.

For Research Use Only. Not For Use In Diagnostic Procedures.

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