Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

Cyclin B1 (V152) Mouse mAb (Alexa Fluor® 488 Conjugate) #4112

Applications Reactivity Sensitivity Isotype
F H M (Hm) Endogenous Mouse IgG1

Applications Key:  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  Hm=Hamster
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Cyclin B1 (V152) Mouse mAb detects endogenous levels of cyclin B1 independent of phosphorylation.

Source / Purification

Cyclin B1 (V152) Monoclonal antibody is produced by immunizing animals with a peptide corresponding to a sequence from hamster cyclin B1. The antibody was conjugated to Alexa Fluor®488 under optimal conditions with an F/P ratio of 2-5.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of untreated Jurkat cells, using Cyclin B1 (V152) (Alexa Fluor®488 Conjugate) Mouse mAb versus propidium iodide (DNA content).

Description

Cell Signaling Technology Antibody conjugated to Alexa Fluor ® 488 fluorescent dye and tested in-house for direct Flow Cytometric analysis of human cells. The unconjugated antibody, #4135, reacts with human and mouse. CST expects that Cyclin B1 (V152) Mouse mAb (Alexa Fluor®488 conjugate) will also recognize Cyclin B1 in these species.

Background

Cyclins are a family of proteins that activate specific cyclin-dependent kinases required for progression through the cell cycle. The entry of all eukaryotic cells into mitosis is regulated by activation of cdc2/cdk1 at the G2/M transition. This activation is a multi-step process that begins with the binding of the regulatory subunit, cyclin B1, to cdc2/cdk1 to form the mitosis-promoting factor (MPF). MPF remains in the inactive state until phosphorylation of cdc2/cdk1 at Thr161 by cdk activating kinase (CAK) (1,2) and dephosphorylation of cdc2/cdk1 at Thr14/Tyr15 by cdc25C (3-5). Four cyclin B1 phosphorylation sites (Ser126, 128, 133, and 147) are located in the cytoplasmic retention signal (CRS) domain and are thought to regulate the translocation of cyclin B1 to the nucleus at the G2/M checkpoint, promoting nuclear accumulation and initiation of mitosis (6-9). While MPF itself can phosphorylate Ser126 and Ser128, polo-like kinase 1 (PLK1) phosphorylates cyclin B1 preferentially at Ser133 and possibly at Ser147 (6,10). At the end of mitosis, cyclin B1 is targeted for degradation by the anaphase-promoting complex (APC), allowing for cell cycle progression (11). Research studies have shown that cyclin B1 is overexpressed in breast, prostate, and non-small cell lung cancers (12-14).

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  9. Takizawa, C.G. and Morgan, D.O. (2000) Curr Opin Cell Biol 12, 658-65.
  10. Jackman, M. et al. (2003) Nat Cell Biol 5, 143-8.
  11. Gong, D. and Ferrell, J.E. (2010) Mol Biol Cell 21, 3149-61.
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  13. Kawamoto, H. et al. (1997) Am J Pathol 150, 15-23.
  14. Soria, J.C. et al. (2000) Cancer Res 60, 4000-4.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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