Product Pathways - Wnt / Hedgehog / Notch
CDCP1 Antibody #4115
| Applications | Reactivity | MW (kDa) | Source |
|---|---|---|---|
| W IP IF-IC | H | 140, 70 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:
H=Human
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
CDCP1 Antibody detects endogenous levels of total CDCP1 protein. The 140kDa band is full length, glycosylated CDCP1, the 70kDa band is a cleavage product of CDCP1.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to amino acids near the carboxy-terminus of human CDCP1. Antibodies are purified by peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from PC3 and HT29 cells using CDCP1 Antibody.
IF-IC
Confocal immunofluorescent analysis of HT-29 cells using CDCP1 Antibody (green). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).
Background
CDCP1 (CUB domain containing protein, SIMA135) is a putative stem cell marker that is highly expressed in some human cancer cells and in both, typical and atypical (cancerous) colons (1). Expression of CDCP1 may be epigenetically regulated, as methylation of promoter CpG sequences results in decreased CDCP1 expression (2). The corresponding CDCP1 gene encodes a glycoprotein that acts as a complex, multidomain transmembrane antigen. Three CUB domains in its extracellular region are possibly involved in cell adhesion or extracellular matrix interaction (1,3). Src-family kinases may phosphorylate CDCP1 at five tyrosine residues within its cytoplasmic domain to provide a potential binding site for SH2- domain containing proteins (3). CDCP1 is a putative marker for hematopoietic stem cells (4,5).
- Scherl-Mostageer, M. et al. (2001) Oncogene 20, 4402-4408.
- Ikeda, J.I. et al. (2006) J. Pathol. 210, 75-84.
- Hooper, J.D. et al. (2003) Oncogene 22, 1783-1794.
- Conze, T. et al. (2003) Ann. N.Y. Acad. Sci. 996, 222-226.
- Buhring, H.J. et al. (2004) Stem Cells 22, 334-343.
Application References
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