Product Pathways - Cell Cycle / Checkpoint
Bub1b Antibody #4116
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP | H | Endogenous | 130 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Bub1b Antibody detects endogenous levels of total Bub1b protein.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to amino acid sequence surrounding Lys370 of human Bub1b. Antibodies are purified by Protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from K562 and NTERA cells using Bub1b Antibody.
Background
Bub1b is a mitotic checkpoint kinase that controls chromosome segregation and monitors kinetochore-microtubule interactions. It is part of the Mitotic Checkpoint Complex (MCC) that also contains Bub1, Bub3, Mad2 and Cdc20 (1). This complex inhibits the ubiquitin ligase activity of the Anaphase Promoting Complex/Cyclosome (APC/C) during mitosis to prevent cells with unaligned chromosomes from prematurely entering anaphase (2). Bub1b and Bub1 are mutated in several types of human malignancies including hematopoietic, colorectal, lung and breast cancers (3). Biallelic mutations in Bub1b have been found in mosaic variegated aneuploidy syndrome and premature chromatid separation syndrome (4). Bub1b mouse germline knockouts are embryonic lethal with heterozygous animals displaying genetic instability, early aging phenotypes and increased cancer susceptibility (5).
- Fukagawa, T. (2008) Front Biosci 13, 2705-13.
- Chen, R.H. (2007) J Biomed Sci 14, 475-9.
- Dai, W. et al. (2004) Cancer Res 64, 440-5.
- Kops, G.J. et al. (2005) Nat Rev Cancer 5, 773-85.
- Baker, D.J. et al. (2004) Nat Genet 36, 744-9.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
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- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
