Product Pathways - Neuroscience
SNAP25 (A195) Antibody #4117
PhosphoSitePlus® protein, site, and accession data: SNAP-25
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | M R (H) | Endogenous | 25 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 4117:
- Western Blotting
Specificity / Sensitivity
SNAP25 (A195) Antibody detects endogenous levels of total SNAP25 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala195 of human SNAP25. Antibodies are purified by protein A and peptide affinity chromatography.
Western blot analysis of extracts from mouse and rat brain using SNAP25 (A195) Antibody.
Background
The 25 kDa synaptosome-associated protein (SNAP25) is a target membrane soluble, N-ethylmaleimide-sensitive factor attachment protein receptor (t-SNARE) that is found on neuronal presynaptic membranes. SNAP25 forms a core complex with the SNARE proteins syntaxin and synaptobrevin to mediate synaptic vesicle fusion with the plasma membrane during Ca2+-dependent exocytosis (1). This complex is responsible for exocytosis of the neurotransmitter γ-aminobutyric acid (GABA). Neurotransmitter release is inhibited by proteolysis of SNAP25 by botulinum toxins A and E (2). SNAP25 plays a secondary role as a Q-SNARE involved in endosome fusion; the protein is associated with genetic susceptibility to attention-deficit hyperactivity disorder (ADHD) (3).
- Salaün, C. et al. (2004) Biochim Biophys Acta 1693, 81-9.
- Fan, H.P. et al. (2006) J Biol Chem 281, 28174-84.
- Aikawa, Y. et al. (2006) Mol Biol Cell 17, 2113-24.
Application References
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
