Product Pathways - Wnt / Hedgehog / Notch
TWIST1 Antibody #4119
| Applications | Reactivity | MW (kDa) | Source |
|---|---|---|---|
| W IP | H Mk | 26 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
Mk=Monkey
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
TWIST1 Antibody detects endogenous levels of total TWIST1 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to amino acids near the carboxy terminus of human TWIST1. Antibodies are purified by peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from NCCIT and SH-SY5Y cells using TWIST1 Antibody.
Background
TWIST1 is a basic helix-loop-helix (b-HLH) transcription factor that functions as a master regulator of embryonic morphogenesis and plays essential roles in mesenchymal differentiation and osteogenic determination (1-3). Mutations affecting the b-HLH domain of the TWIST1 gene have been associated with Saethre-Chotzen syndrome, an autosomal dominant craniosynostosis disorder causing craniofacial and limb abnormalities (4,5). TWIST1 is upregulated in various human tumors and may play a role in EMT (epithelial-mesenchymal transition) and metastasis (6,7). Upregulation of TWIST1 may contribute to resistance to Taxol and microtubule regulating drugs in tumors (8).
- Thisse, B. et al. (1987) Nucleic Acids Res. 15, 3439-3453.
- Lee, M.S. et al. (1999) J. Cell Biochem. 75, 566-577.
- Chen, Z.F. and Behringer, R.R. (1995) Genes Dev. 9, 686-699.
- Howard, T.D. et al. (1997) Nat. Genet. 15, 36-41.
- Connerney, J. et al. (2006) Dev. Dyn. 235, 1345-1357.
- Watanabe, O. et al. (2004) Anticancer Res. 24, 3851-3856.
- Yang, J. et al. (2004) Cell 117, 927-939.
- Wang, X. et al. (2004) Oncogene 23, 474-482.
Application References
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