Product Pathways - Cell Cycle / Checkpoint
Phospho-TIF1β (Ser824) Antibody #4127
Reactivity Key: H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
Phospho-TIF1β (Ser824) Antibody detects endogenous levels of TIF1β protein only when phosphorylated on Ser824.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to amino acids surrounding Ser824 of human TIF1β. Antibodies are purified by peptide affinity chromatography.
TIF1β is a member of the TIF1 (transcriptional intermediary factor 1) family, a group of transcriptional regulators that play key roles in development and differentiation. Members of this family are characterized by the presence of two conserved motifs – an N-terminal RING-B box-coiled-coil motif and a C-terminal PHD finger and bromodomain unit (1,2). TIF1β is a corepressor for KRAB (Kruppel associated box) domain containing zinc finger proteins. The KRAB domain containing zinc finger proteins are a large group of transcription factors that are vertebrate-specific, varied in their expression patterns between species, and thought to regulate gene transcription programs that control speciation (3,4).TIF1β has been shown to be essential for early embryonic development and spermatogenesis (6,5). It functions to either activate or repress transcription in response to environmental or developmental signals by chromatin remodeling and histone modification. The recruitment and association of TIF1β with heterochromatin protein (HP1) is essential for transcriptional repression, and for progression through differentiation of F9 embryonic carcinoma cells (6,7). TIF1β also plays a role in the DNA damage response. Phosphorylation of TIF1β on Ser842 occurs in an ATM-dependent manner in response to genotoxic stress and is thought to be essential for chromatin relaxation, which is in turn required for the DNA damage response (8).
- Le Douarin, B. et al. (1995) EMBO J. 14, 2020-2033.
- Le Douarin, B. et al. (1996) EMBO J. 15, 6701-6715.
- Friedman, J.R. et al. (1996) Genes Dev. 10, 2067-2078.
- Krebs, C.J. et al. (2005) Genomics 85, 752-761.
- Weber, P. et al. (2002) Development 129, 2329-2337.
- Cammas, F. et al. (2004) Genes Dev. 18, 2147-2160.
- Cammas, F. et al. (2007) Differentiation 75, 627-37.
- Ziv, Y. et al. (2006) Nat. Cell Biol. 8, 870-876.
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For Research Use Only. Not For Use In Diagnostic Procedures.