Product Pathways - Cell Cycle / Checkpoint
Phospho-Cyclin B1 (Ser147) Antibody #4131
|W||H M R (Pg)||Endogenous||58||Rabbit|
Reactivity Key: H=Human M=Mouse R=Rat Pg=Pig
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
Phospho-Cyclin B1 (Ser147) Antibody detects endogenous levels of cyclin B1 only when phosphorylated at serine 147.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser147 of human cyclin B1. Antibodies are purified by protein A and peptide affinity chromatography.
Western blot analysis of extracts from 293 cells, using Phospho-Cyclin B1 (Ser147) Antibody (A) and Cyclin B1 (V152) Monoclonal Antibody (C). Treatment of the membrane with calf intestinal alkaline phosphatase (CIP) after Western transfer abolishes the phospho-cyclin B1 signal (B), but has no effect on the total cyclin B1 signal (D).
Western blot analysis of extracts from HT29 cells, untreated (cont.) or treated with 50 ng/ml nocodazole (M), using Phospho-cyclin B1 (Ser147) Antibody (upper), Cyclin B1 (V152) Monoclonal Antibody (middle), or cdc25C Antibody #9522 (lower).
Western blot analysis of extracts from asynchronous cells (lane 1), S-phase and G2/M blocked cells (lane 2), or synchronized cells collected at various time points following release from this block (lanes 3-7) using Phospho-Cyclin B1 (Ser147) Antibody (upper). Cell cycle synchronization was verified by flow cytometric analysis of DNA content (lower). (Data kindly provided by Ethan Kohn and Alan Eastman, Dartmouth Medical School, Hanover, NH).
Cyclins are a family of proteins that activate specific cyclin-dependent kinases required for progression through the cell cycle. The entry of all eukaryotic cells into mitosis is regulated by activation of cdc2/cdk1 at the G2/M transition. This activation is a multi-step process that begins with the binding of the regulatory subunit, cyclin B1, to cdc2/cdk1 to form the mitosis-promoting factor (MPF). MPF remains in the inactive state until phosphorylation of cdc2/cdk1 at Thr161 by cdk activating kinase (CAK) (1,2) and dephosphorylation of cdc2/cdk1 at Thr14/Tyr15 by cdc25C (3-5). Four cyclin B1 phosphorylation sites (Ser126, 128, 133, and 147) are located in the cytoplasmic retention signal (CRS) domain and are thought to regulate the translocation of cyclin B1 to the nucleus at the G2/M checkpoint, promoting nuclear accumulation and initiation of mitosis (6-9). While MPF itself can phosphorylate Ser126 and Ser128, polo-like kinase 1 (PLK1) phosphorylates cyclin B1 preferentially at Ser133 and possibly at Ser147 (6,10). At the end of mitosis, cyclin B1 is targeted for degradation by the anaphase-promoting complex (APC), allowing for cell cycle progression (11). Research studies have shown that cyclin B1 is overexpressed in breast, prostate, and non-small cell lung cancers (12-14).
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For Research Use Only. Not For Use In Diagnostic Procedures.