Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

Cyclin B1 (V152) Mouse mAb #4135

Applications Reactivity Sensitivity MW (kDa) Isotype
W F H M (Hm) Endogenous 60 Mouse IgG1

Applications Key:  W=Western Blotting  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  Hm=Hamster
Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Cyclin B1 (V152) Mouse mAb detects endogenous levels of cyclin B1 independent of phosphorylation.

Source / Purification

Monoclonal antibody was produced by immunizing mice with a peptide corresponding to a sequence from hamster cyclin B1.

Western Blotting

Western Blotting

Western blot analysis of extracts from HT29 cells, control (cont.) or nocodazole-treated (50 ng/ml) (M), using Phospho-cyclin B1 (Ser147) Antibody #4131 (upper), Cyclin B1 (V152) Mouse mAb (middle), or Cdc25C Antibody #9522 (lower).

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of untreated Jurkat cells, using Cyclin B1 (V152) Mouse mAb versus propidium iodide (DNA content). The box indicates Cyclin B1-positive cells.

Background

Entry of all eukaryotic cells into mitosis is regulated by activation of cdc2 kinase. Activation of cdc2 is controlled at several steps including cyclin B1 binding, phosphorylation of cdc2 at Thr161 and dephosphorylation of cdc2 at Thr14/Tyr15 (1-5). The protein levels of CDK inhibitors and the CDK-associated cyclins are regulated by phosphorylation, ubiquitination and degradation, allowing for a stoichiometric regulation of cell cycle events (6). Four cyclin B1 phosphorylation sites (Ser126, 128, 133 and 147) are located in the cytoplasmic retention signal (CRS) domain and are thought to regulate the translocation of cyclin B1 to the nucleus at the G2/M checkpoint (8-10). Phosphorylation of cyclin B1 is required for cdc25C-dependent dephosphorylation of Tyr15 within cdc2 and subsequent cdc2/cyclin B1 activation (11). While cdc2/cyclin B1 itself can phosphorylate Ser126 and Ser128 (8), polo-like kinase 1 phosphorylates cyclin B1 preferentially at Ser133 and possibly at Ser147 as well (11-13).

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  2. Atherton-Fessler, S. et al. (1993) Mol. Cell. Biol. 13, 1675-1685.
  3. Watanabe, N. et al. (1995) EMBO J. 14, 1878-1891.
  4. Galaktionov, K. et al. (1995) Genes Dev. 9, 1046-1058.
  5. Hunter, T. et al. (1995) Cell 80, 225-236.
  6. Diehl, J.A. et al. (1997) Genes Dev. 11, 957-972.
  7. McGowan, C.H. et al. (1993) EMBO J. 12, 75-85.
  8. Izumi, T. et al. (1991) Mol. Cell. Biol. 11, 3860-3867.
  9. Li, J. et al. (1995) Mol. Biol. Cell 6, 1111-1124.
  10. Li, J. et al. (1997) Proc. Natl. Acad. Sci. USA 94, 502-507.
  11. Toyoshima-Morimoto, F. et al. (2001) Nature 410, 215-220.
  12. Peter, M. et al. (2002) EMBO Rep. 3, 551-556.
  13. Jackman, M. et al. (2003) Nat. Cell Biol. 5, 143-148.

Application References

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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.

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