Product Pathways - Cell Cycle / Checkpoint
Phospho-Cyclin E1 (Thr62) Antibody #4136
|4136S||100 µl (10 western blots)||---||In Stock||---|
|4136||carrier free and custom formulation / quantity||email request|
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Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), F=Flow Cytometry
Specificity / Sensitivity
Phospho-Cyclin E1 (Thr62) Antibody detects endogenous levels of cyclin E only when phosphorylated at threonine 62 (cyclin E1 isoform 2) or threonine 77 (cyclin E1 isoform 1).
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr62 of human cyclin E1. Antibodies are purified by protein protein A and peptide affinity chromatography.
Western blot analysis of extracts from 293 cells, treated with 10 mM LiCl for the indicated times, using Phospho-Cyclin E1 (Thr62) Antibody (upper) or a cyclin E antibody (lower).
Western blot analysis of extracts from 293 cells, untreated or hydroxyurea-treated, using Phospho-Cyclin E1 (Thr62) Antibody.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, showing nuclear localization, using Phospho-Cyclin E1 (Thr62) Antibody.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Phospho-Cyclin E1 (Thr62) Antibody.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Phospho-Cyclin E1 (Thr62) Antibody in the presence of control peptide (left) or antigen-specific peptide (right).
Flow cytometric analysis of untreated Jurkat cells, using Phospho-Cyclin E1 (Thr62) Antibody versus propidium iodide (DNA content). The boxed population indicates phospho-Cyclin E (Thr62)-positive cells.
Cyclin E1 and cyclin E2 can associate with and activate CDK2 (1). Upon DNA damage, upregulation/activation of the CDK inhibitors p21 Waf1/Cip1 and p27 Kip1 prevent cyclin E/CDK2 activation, resulting in G1/S arrest. When conditions are favorable for cell cycle progression, cyclin D/CDK4/6 phosphorylates Rb and is thought to reduce the activity of p21 Waf1/Cip1 and p27 Kip1, allowing subsequent activation of cyclin E/CDK2 (1,2). Cyclin E/CDK2 further phosphorylates Rb to allow progression into S-phase, where cyclin E/CDK2 is thought to phosphorylate and activate multiple proteins involved in DNA synthesis (2,3). Turnover of cyclin E is largely controlled by phosphorylation that results in SCFFbw7-mediated ubiquitination and proteasome-dependent degradation (4,5). Cyclin E1 is phosphorylated at multiple sites in vivo including Thr62, Ser88, Ser72, Thr380 and Ser384, and is controlled by at least two kinases, CDK2 and GSK-3 (6,7).
- Lauper, N. et al. (1998) Oncogene 17, 2637-2643.
- Lundberg, A.S. and Weinberg, R.A. (1998) Mol. Cell. Biol. 18, 753-761.
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- Won, K.A. and Reed, S.I. (1996) EMBO J. 15, 4182-4193.
- Koepp, D.M. et al. (2001) Science 294, 173-177.
- Welcker, M. et al. (2003) Mol. Cell 12, 381-392.
- Ye, X. et al. (2004) J. Biol. Chem. 279, 50110-50119.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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