Product Pathways - Cytoskeletal Signaling
GEF-H1 Antibody #4145
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H M R Mk B | Endogenous | 120 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
B=Bovine
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
GEF-H1 Antibody recognizes endogenous levels of total GEF-H1 protein. In human and monkey cells, the antibody cross-reacts with an unrelated 70 kDa protein.
Source / Purification
Antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to the carboxy-terminus of human GEF-H1. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from 293, NIH/3T3, C6 and COS cells, using GEF-H1 Antibody.
Background
Rho family small GTPases regulate processes such as cell migration, adhesion, proliferation and differentiation. They are activated by guanine nucleotide exchange factors (GEFs), which catalyze the exchange of GDP for GTP. GEF-H1 is a Rho GEF that localizes to microtubules and regulates Rho activity in response to microtubule destabilization (1). Loss of interaction between GEF-H1 and microtubules leads to activation of Rho (2). PAK1 phosphorylates GEF-H1 at Ser885, a site located in the 14-3-3 binding motif. Phosphorylation at this site is required for 14-3-3 binding and correlated to recruitment of 14-3-3 and GEF-H1 to microtubules (3).GEF-H1 has also been shown to localize to tight junctions and modulate polarized cell permeability (4,5). GEF-H1 is inactivated by binding to cingulin at epithelial tight junctions, inactivating RhoA and leading to G1/S arrest (5).
- Ren, Y. et al. (1998) J. Biol. Chem. 273, 34954-34960.
- Krendel, M. et al. (2002) Nat. Cell Biol. 4, 294-301.
- Zenke, F. T. et al. (2004) J. Biol. Chem. 279, 18392-18400.
- Benais-Pont, G. et al. (2003) J. Cell Biol. 160, 729-740.
- Aijaz, S. et al. (2005) Dev. Cell 8, 777-786.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 4076 GEF-H1 (55B6) Rabbit mAb
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
