Product Pathways - Cytoskeletal Signaling
GEF-H1 Antibody #4145
|W||H M R Mk B||Endogenous||120||Rabbit|
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey B=Bovine
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
GEF-H1 Antibody recognizes endogenous levels of total GEF-H1 protein. In human and monkey cells, the antibody cross-reacts with an unrelated 70 kDa protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy-terminus of human GEF-H1. Antibodies are purified by protein A and peptide affinity chromatography.
Rho family small GTPases regulate processes such as cell migration, adhesion, proliferation and differentiation. They are activated by guanine nucleotide exchange factors (GEFs), which catalyze the exchange of GDP for GTP. GEF-H1 is a Rho GEF that localizes to microtubules and regulates Rho activity in response to microtubule destabilization (1). Loss of interaction between GEF-H1 and microtubules leads to activation of Rho (2). PAK1 phosphorylates GEF-H1 at Ser885, a site located in the 14-3-3 binding motif. Phosphorylation at this site is required for 14-3-3 binding and correlated to recruitment of 14-3-3 and GEF-H1 to microtubules (3).GEF-H1 has also been shown to localize to tight junctions and modulate polarized cell permeability (4,5). GEF-H1 is inactivated by binding to cingulin at epithelial tight junctions, inactivating RhoA and leading to G1/S arrest (5).
- Ren, Y. et al. (1998) J. Biol. Chem. 273, 34954-34960.
- Krendel, M. et al. (2002) Nat. Cell Biol. 4, 294-301.
- Zenke, F. T. et al. (2004) J. Biol. Chem. 279, 18392-18400.
- Benais-Pont, G. et al. (2003) J. Cell Biol. 160, 729-740.
- Aijaz, S. et al. (2005) Dev. Cell 8, 777-786.
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For Research Use Only. Not For Use In Diagnostic Procedures.