Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

ADAM9 (D64B5) Rabbit mAb #4151

Applications Reactivity Sensitivity MW (kDa) Isotype
W H M R Mk Endogenous 100-115, 75-80 Rabbit IgG

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

ADAM9 (D64B5) Rabbit mAb detects endogenous levels of total ADAM9 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human ADAM9.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using ADAM9 (D64B5) Rabbit mAb.

Background

The ADAM (A Disintegrin and A Metalloprotease) family of multidomain membrane proteins influences cell signaling and adhesion by shedding cell surface proteins such as cytokines and growth factors, by influencing cell adhesion to the extracellular matrix (ECM), and by directly remodeling the ECM. Conserved domains in ADAM family members include a prodomain, a zinc-dependent metalloprotease domain, a disintegrin domain, a cysteine-rich domain, an EGF-like sequence, and a short cytoplasmic tail (1,2).The prodomain is thought to aid in protein folding. Disintegrin and cysteine-rich domains mediate adhesion, at least in part, through binding to integrins. Phosphorylation of the cytoplasmic tail as well as its interaction with other signaling proteins may influence intra- and extracellular signaling (1). ADAM9 is widely distributed and has been shown to affect migration in skin keratinocytes (3,4). Research studies have shown that ADAM9 is overexpressed in prostate cancer (5), pancreatic cancer (6), gastric cancer (7), and has been linked to invasion and metastasis in small cell lung cancer (8). Research has also shown that an alternatively spliced short (50 kDa) form of ADAM9 containing protease activity is involved in tumor cell invasion (9).

  1. N. M. Hooper and U. Lendeckel. The Adam Family Of Proteases. The Netherlands: Springer, 2005
  2. Schlöndorff, J. and Blobel, C.P. (1999) J Cell Sci 112 ( Pt 21), 3603-17.
  3. Franzke, C.W. et al. (2002) EMBO J 21, 5026-35.
  4. Zigrino, P. et al. (2007) J Biol Chem 282, 30785-93.
  5. Fritzsche, F.R. et al. (2008) Eur Urol 54, 1097-106.
  6. Grützmann, R. et al. (2004) Br J Cancer 90, 1053-8.
  7. Carl-McGrath, S. et al. (2005) Int J Oncol 26, 17-24.
  8. Shintani, Y. et al. (2004) Cancer Res 64, 4190-6.
  9. Mazzocca, A. et al. (2005) Cancer Res 65, 4728-38.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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