Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

EPAC1 (5D3) Mouse mAb #4155

Applications Reactivity Sensitivity MW (kDa) Isotype
W H M R Endogenous 100 Mouse IgG2a

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Epac1 (5D3) Mouse mAb detects endogenous levels of total Epac1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant fragment of human Epac1.

Western Blotting

Western Blotting

Western blot analysis of extracts from C2C12 cells and whole rat brain using Epac1 (5D3) Mouse mAb.

Background

EPAC1 and EPAC2 (exchange proteins activated by cyclic AMP) are guanine nucleotide exchange factors (GEFs) that catalyze the exchange of GDP for GTP, activating Rap1 and Rap2 small GTPases. Rap activation by EPAC is cAMP-dependent and mediates cAMP signaling in part through protein kinase A (PKA) (reviewed in 1). EPAC signaling plays a significant role in a number of cellular processes including migration and focal adhesion formation (2), exocytosis (3), insulin signaling (4), axon growth and guidance (5) and neurotransmitter release (6).

  1. Bos, J.L. (2006) Trends Biochem Sci 31, 680-6.
  2. Lyle, K.S. et al. (2008) Cell Signal 20, 1104-16.
  3. Branham, M.T. et al. (2009) J Biol Chem 284, 24825-39.
  4. Petersen, R.K. et al. (2008) Mol Cell Biol 28, 3804-16.
  5. Murray, A.J. and Shewan, D.A. (2008) Mol Cell Neurosci 38, 578-88.
  6. Ouyang, M. et al. (2008) Proc Natl Acad Sci USA 105, 11993-7.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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