Cell Signaling Technology

Product Pathways - Translational Control

DHX29 Antibody #4159

Applications Reactivity Sensitivity MW (kDa) Source
W IP H M R Endogenous 155 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

DHX29 Antibody detects endogenous levels of total DHX29 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues at the C-terminus of human DHX29. Antibodies were purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa and HepG2 cells using DHX29 Antibody.

Background

DHX29 is an ATP-dependent RNA helicase that belongs to the DEAD-box helicase family (DEAH subfamily). DHX29 contains one central helicase and one helicase at the carboxy-terminal domain (1). Its function has not been fully established but DHX29 was recently shown to facilitate translation initiation on mRNAs with structured 5' untranslated regions (2). DHX29 binds 40S subunits and hydrolyzes ATP, GTP, UTP, and CTP. Hydrolysis of nucleotide triphosphates by DHX29 is strongly stimulated by 43S complexes and is required for DHX29 activity in promoting 48S complex formation (2).

  1. de la Cruz, J. et al. (1999) Trends Biochem Sci 24, 192-8.
  2. Pisareva, V.P. et al. (2008) Cell 135, 1237-50.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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