Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

Myeloperoxidase (P733) Antibody #4163

Applications Reactivity Sensitivity MW (kDa) Source
W H R (Mk) Endogenous 60, 80-90 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Myeloperoxidase (P733) Antibody detects endogenous levels of total myeloperoxidase. The antibody detects the heavy chain of myeloperoxidase.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro733 of human myeloperoxidase. Antibodies were purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of recombinant MPO (50 ng) using Myeloperoxidase (P733) Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Myeloperoxidase (P733) Antibody. HL-60 cells were treated with TPA #9905 (200 nM, overnight).

Background

Myeloperoxidase (MPO) is a peroxidase enzyme that is part of the host defense system of polymorphonuclear leukocytes (reviewed in 1). The gene for MPO was cloned independently from several laboratories (2-5). A decrease in MPO expression was noticed upon differentiation of HL-60 cells (5). MPO catalyzes the reaction of hydrogen peroxide and chloride (or other halides) to produce hypochlorous acid and other potent antimicrobial oxidants. Knockout mice of MPO are impaired in clearing select microbial infections (6). Processing of mature MPO from an initial 80-90 kDa translation product involves insertion of a heme moiety, glycosylation, and proteolytic cleavage. The mature protein is a tetramer of two heavy chains (60 kDa) and two light chains (12 kDa). It is abundantly expressed in neutrophils and monocytes and secreted during their activation. Heightened MPO levels have been associated with tissue damage and a number of pathological conditions (1).

  1. Klebanoff, S.J. (2005) J Leukoc Biol 77, 598-625.
  2. Chang, K.S. et al. (1986) Blood 68, 1411-4.
  3. Yamada, M. et al. (1987) Arch Biochem Biophys 255, 147-55.
  4. Morishita, K. et al. (1987) J Biol Chem 262, 3844-51.
  5. Weil, S.C. et al. (1987) Proc Natl Acad Sci USA 84, 2057-61.
  6. Aratani, Y. et al. (2002) J Infect Dis 185, 1833-7.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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