Product Pathways - Chromatin Regulation
TFIIB (2F6A3H4) Mouse mAb #4169
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP | H M R Mk | Endogenous | 32 | Mouse IgG2b |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by Western blot.
Protocols
Specificity / Sensitivity
TFIIB (2F6A3H4) Mouse mAb detects endogenous levels of total TFIIB protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant human TFIIB protein.
Western Blotting
Western blot analysis of extracts from various cell lines using TFIIB (2F6A3H4) Mouse mAb.
Background
TFIIB (also known as GTF2B, TF2B and general transcription factor IIB) is a ubiquitously expressed transcription factor that plays a central role in the assembly of the transcription pre-initiation complex through direct recruitment of RNA polymerase II (1,2). TFIIB functions as a bridge between promoter-bound TFIID and RNA polymerase II. In addition to interacting with promoter-bound TFIID and TFIIA, TFIIB makes extensive contacts with the core promoter via two independent DNA-binding modules. TFIIB may also be a target of transcriptional activator proteins that act to stimulate pre-initiation complex assembly.
- Deng, W. and Roberts, S.G. (2007) Chromosoma 116, 417-29.
- Thomas, M.C. and Chiang, C.M. Crit Rev Biochem Mol Biol 41, 105-78.
Application References
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
