Product Pathways - MAPK Signaling
Phospho-MKK7 (Ser271/Thr275) Antibody #4171
|4171S||100 µl (10 western blots)||---||In Stock||---|
|4171||carrier free and custom formulation / quantity||email request|
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Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Species predicted to react based on 100% sequence homology: Mouse, Rat.
Specificity / Sensitivity
Phospho-MKK7 (Ser171/Thr275) Antibody detects endogenous levels of MKK7 phosphorylated at serine 271 and threonine 275. This antibody does not cross-react with phosphorylated SEK1/MKK4 or other phosphorylated MAP kinase kinases.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser271/Thr275 of human MKK7. Antibodies are purified by protein A and peptide affinity chromatography.
MKK7 is a MAP kinase kinase that serves as a specific activator of the SAPK/JNK pathway (1,2). MKK7 is strongly activated by TNF-α, as well as other environmental stresses, whereas SEK1/MKK4, which activates both p38 and SAPK/JNK pathways, is not activated by TNF-α (2). Sequence alignment of the activation loop of the MAP kinase kinase family members indicates that Ser271 and Thr275 are potential phosphorylation sites that are crucial for the kinase acivity.
- Zapata, H.J. et al. (2007) J Virol 81, 977-90. Applications: Western Blotting.
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