Cell Signaling Technology

Product Pathways - MAPK Signaling

Phospho-MKK7 (Ser271/Thr275) Antibody #4171

Applications Reactivity Sensitivity MW (kDa) Source
W H (M) (R) Endogenous 48 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Phospho-MKK7 (Ser171/Thr275) Antibody detects endogenous levels of MKK7 phosphorylated at serine 271 and threonine 275. This antibody does not cross-react with phosphorylated SEK1/MKK4 or other phosphorylated MAP kinase kinases.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser271/Thr275 of human MKK7. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis and extracts from THP1, U-937 and Ramos cells, untreated or sorbitol-treated (400mM, 30 min), using Phospho-MKK7 (Ser271/Thr275) Antibody (upper) or MKK7 Antibody #4172 (lower).

Background

MKK7 is a MAP kinase kinase that serves as a specific activator of the SAPK/JNK pathway (1,2). MKK7 is strongly activated by TNF-α, as well as other environmental stresses, whereas SEK1/MKK4, which activates both p38 and SAPK/JNK pathways, is not activated by TNF-α (2). Sequence alignment of the activation loop of the MAP kinase kinase family members indicates that Ser271 and Thr275 are potential phosphorylation sites that are crucial for the kinase acivity.

  1. Tournier, C. et al. (1999) Mol. Cell. Biol. 19, 1569-1581.
  2. Moriguchi, T. et al. (1997) EMBO J. 16, 7045-7053.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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