Cell Signaling Technology

Product Pathways - Ca / cAMP / Lipid Signaling

TPA (12-O-Tetradecanoylphorbol-13-Acetate) #4174

Molecular Formula:
C36H56O8
Molecular Weight:
616.84 g/mol
Purity:
>99.5%

Directions for Use

TPA is supplied as a 1 mg powder. Store at -20C. Before use, dissolve powder in 1 ml DMSO to yield a 1.6 mM stock solution. For working concentration of 200 μM, add 125 μl stock solution to 875 μl DMSO. Treat cells with 200 nM TPA for 20-30 minutes.

Western Blotting

Western Blotting

Western blot analysis of extracts from serum-starved 293 cells, untreated (lane 1), stimulated with TPA treatment (lanes 2 & 3), and treated with CIP and λ phosphatase (lane 3), using Phospho-PKC (pan) (βII Ser660) Antibody #9371 or PKCα Antibody #2056.

Western Blotting

Western Blotting

Western blot analysis of extracts from serum-starved 293 cells, untreated (lane 1), stimulated with TPA treatment (lanes 2 & 3), and treated with CIP and λ phosphatase (lane 3), using Phospho-PKD/PKCμ (Ser916) Antibody #2051 or PKD/PKCμ Antibody #2052.

Description

TPA (12-O-tetradecanoylphorbol-13-acetate)/ PMA (phorbol-12-myristate-13-acetate) is soluble in DMSO and ethanol. DMSO solutions can be stored at -20C, in the absence of light, for at least six months. A 200 nM TPA treatment is standard to induce signaling.

Background

TPA (12-O-tetradecanoylphorbol-13-acetate) / PMA (phorbol-12-myristate-13-acetate) is the most commonly used phorbol ester. It binds and activates protein kinase C, causing a wide range of effects in cells and tissues (1,2). TPA has been demonstrated to be a potent tumor promoter in mouse skin (3). Conversely, it has been shown to be an effective cancer therapeutic agent in myelocytic leukemia patients, and has been indicated as a potential colorectal cancer therapeutic (4-5). TPA has also been shown to increase white blood cell and neutrophil counts in solid tumor cancer patients (6).

  1. Rebois, R.V. and Patel, J. (1985) J Biol Chem 260, 8026-31.
  2. Blumberg, P.M. (1980) Crit Rev Toxicol 8, 153-97.
  3. Fürstenberger, G. et al. (1981) Proc Natl Acad Sci U S A 78, 7722-6.
  4. Han, Z.T. et al. (1998) Proc Natl Acad Sci U S A 95, 5357-61.
  5. Lee, H.W. et al. (2002) J Biol Chem 277, 32624-31.
  6. Han, Z.T. et al. (1998) Proc Natl Acad Sci U S A 95, 5362-5.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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