Cell Signaling Technology
XP Monoclonal Antibody

Product Pathways - Neuroscience

α-Synuclein (D37A6) XP® Rabbit mAb #4179

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP IHC-P IF-F M R Endogenous 18 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)  IF-F=Immunofluorescence (Frozen)
Reactivity Key:  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

α-Synuclein (D37A6) XP® Rabbit mAb detects endogenous levels of the α isoform of synuclein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide surrounding Glu105 of mouse α-synuclein protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from mouse and rat brain using α-Synuclein (D37A6) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse brain using α-Synuclein (D37A6) XP® Rabbit mAb.

IF-F

IF-F

Confocal immunofluorescent analysis of normal rat cerebellum, hippocampus and striatum using α-Synuclein (D37A6) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).


Background

α-Synuclein is a protein of 140-amino acids expressed abundantly in the brain. α-Synuclein is also the main component of pathogenic Lewy bodies and Lewy neurites. Research studies have shown that mutations of the α-synuclein gene are linked to Parkinson's disease (1).

  1. Goldberg, M.S. and Lansbury Jr., P.T. (2000) Nat. Cell Biol. 2, 115-119.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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