Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Phospho-Stathmin (Ser38) (D19H10) Rabbit mAb #4191

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP IHC-P IF-IC H Mk Endogenous 19, 20 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Phospho-Stathmin (Ser38) (D19H10) Rabbit mAb detects endogenous levels of stathmin protein only when phosphorylated at Ser38.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser38 of human stathmin protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from HT-29 cells, untreated or treated with nocodazole alone (100 ng/mL 24 hours) or nocodazole followed by λ and calf intestinal phosphatases, using Phospho-Stathmin (Ser38) (D19H10) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from HT-29 and U-2 OS cells, untreated or synchronized in mitosis, using Phospho-Stathmin (Ser38) (D19H10) Rabbit mAb. Mitotic synchrony was performed by using either a thymidine block followed by release into 100 ng/mL nocodazole for 24 hours or using 100 ng/mL Docetaxel #9886 for 24 hours.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma control (left) or λ phosphatase-treated (right) using Phospho-Stathmin (Ser38) (D19H10) Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded HT-29 cell pellets, untreated (left) or nocodazole-treated (right), using Phospho-Stathmin (Ser38) (D19H10) Rabbit mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of HT-29 cells, untreated (left) or λ phosphatase-treated (right), using Phospho-Stathmin (Ser38) (D19H10) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Background

Stathmin is a ubiquitously expressed microtubule destabilizing phosphoprotein that is upregulated in a number of cancers. The amino terminus of the protein contains multiple phosphorylation sites and is involved in the promotion of tubulin filament depolymerization. Phosphorylation at these sites inactivates the protein and stabilizes microtubules. Ser16 phosphorylation by CaM kinases II and IV (1,2) increases during G2/M-phase and is involved in mitotic spindle regulation (3,4). Ser38 is a target for cdc2 kinase (5) and TNF-induced cell death gives rise to reactive oxygen intermediates leading to hyperphosphorylation of stathmin (6). EGF receptor activation of Rac and cdc42 also increases phosphorylation of stathmin on Ser16 and Ser38 (7). Other closely related family members are neuronally expressed and include SCG10, SCLIP, RB3 and its splice variants RB3' and RB3''. Stathmin and SCG10 have been shown to play roles in neuronal-like development in PC12 cells (8).

  1. Marklund, U. et al. (1994) Eur. J. Biochem. 225, 53-60.
  2. le Gouvello, S. et al. (1998) J. Immunol. 161, 1113-1133.
  3. Mistry, S.J. and Atweh, G.F. (2001) J. Biol. Chem. 276, 31209-31215.
  4. Gavet, O. et al. (1998) J. Cell Sci. 111, 3333-3346.
  5. Luo, X. N. et al. (1994) J. Biol. Chem. 269, 10312-10318.
  6. Vancompernolle, K. et al. (2000) J. Biol. Chem. 275, 33876-33882.
  7. Daub, H. et al. (2001) J. Biol. Chem. 276, 1677-1680.
  8. Di Paolo, G. et al. (1996) J. Cell. Biol. 133, 1383-1390.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Products