Cell Signaling Technology

Product Pathways - Development

Cripto (D81B12) Rabbit mAb (Human Specific) #4193

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H Endogenous 20 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Cripto (D81B12) Rabbit mAb (Human Specific) detects endogenous levels of total cripto protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to an amino acid sequence at the N-terminus of human cripto.

Western Blotting

Western Blotting

Western blot analysis of extracts of NCCIT and NTERA-2 cells using Cripto (D81B12) Rabbit mAb (Human Specific).

Background

Cripto, also known as teratocarcinoma derived growth factor 1 (TDGF-1), belongs to the EGF-CFC family of proteins. Members of this family are characterized by an N-terminal signal peptide, a conserved cysteine rich domain (CFC motif), and a short hydrophobic carboxy-terminal tail that contains GPI cleavage and attachment sites. The GPI moiety anchors Cripto and family members to the extracellular plasma membrane (1). An O-linked fucosylation site within the EGF-like motif is required for Cripto and related family members to perform their function as co-receptors for TGF-β-related ligands such as Nodal and Vg1/GDF1 (2,3). Soluble forms of Cripto can be produced - these contain intact EGF and CFC domains, and are thought to have paracrine activities, as opposed to the autocrine activity of Cripto functioning as a coreceptor (4). Understanding of this paracrine activity is not complete, but it is proposed that Cripto may act as co-ligand for Nodal (3).Cripto is an important modulator of embryogenesis and oncogenesis (4). It is highly expressed in early embryos, and in embryonic stem (ES) cells where it is involved in cardiomyocytic differentiation and acts as a negative regulator of neurogenesis (5-7). Transient activation of Cripto is essential for the capacity of stem cell self-renewal and pluripotency in ES cells, and in some adult derived stem cells (8). Signaling through Cripto can also stimulate other activities that promote tumorigenesis such as stimulation of proliferation, cell motility, invasion, angiogenesis and epithelial-mesenchymal transition (EMT) (9-11). Cripto is highly expressed in a broad range of tumors, where it acts as a potent oncogene.

  1. Minchiotti, G. et al. (2000) Mech Dev 90, 133-42.
  2. Schiffer, S.G. et al. (2001) J Biol Chem 276, 37769-78.
  3. Yan, Y.T. et al. (2002) Mol Cell Biol 22, 4439-49.
  4. Strizzi, L. et al. (2005) Oncogene 24, 5731-41.
  5. Xu, C. et al. (1998) Dev Biol 196, 237-47.
  6. Parisi, S. et al. (2003) J Cell Biol 163, 303-14.
  7. Minchiotti, G. (2005) Oncogene 24, 5668-75.
  8. Beachy, P.A. et al. (2004) Nature 432, 324-31.
  9. Wechselberger, C. et al. (2001) Exp Cell Res 266, 95-105.
  10. Bianco, C. et al. (2005) J Natl Cancer Inst 97, 132-41.
  11. Strizzi, L. et al. (2004) J Cell Physiol 201, 266-76.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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