Product Pathways - Neuroscience
NMDAR1 Antibody #4204
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP | H M R | Endogenous | 120 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
NMDAR1 Antibody detects endogenous levels of total NMDAR1 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to human NMDAR1. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from U87-MG and SH-SY5Y cells, using NMDAR1 Antibody.
Background
N-methyl-D-aspartate receptor (NMDAR) forms a heterodimer of at least one NR1 and one NR2A-D subunit. Multiple receptor isoforms with distinct brain distributions and functional properties arise by selective splicing of the NR1 transcripts and differential expression of the NR2 subunits. NR1 subunits bind the co-agonist glycine and NR2 subunits bind the neurotransmitter glutamate. Activation of the NMDA receptor or opening of the ion channel allows flow of Na+ and Ca2+ ions into the cell, and K+ out of the cell (1). Each subunit has a cytoplasmic domain that can be directly modified by the protein kinase/phosphatase (2). PKC can phosphorylate the NR1 subunit (NMDAR1) of the receptor on Ser890/Ser896, and PKA can phosphorylate NR1 on Ser897 (3). The phosphorylation of NR1 by PKC decreases its affinity for calmodulin, thus preventing the inhibitory effect of calmodulin on NMDAR (4). The phosphorylation of NR1 by PKA probably counteracts the inhibitory effect of calcineurin on the receptor (5). NMDAR mediates long-term potentiation and slow postsynaptic excitation, which play central roles in learning, neurodevelopment and neuroplasticity (6).
- Liu, X.B. et al. (2004) J. Neurosci. 24, 8885-8895.
- Westphal, R.S. et al. (1999) Science 285, 93-96.
- Tingley, W.G. et al. (1997) J. Biol. Chem. 272, 5157-5166.
- Hisatsune, C. et al. (1997) J. Biol. Chem. 272, 20805-20810.
- Raman, I.M. et al. (1996) Neuron 16, 415-421.
- Makhinson, M. et al. (1999) J. Neurosci. 19, 2500-2510.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 3381 Phospho-NMDAR1 (Ser890) Antibody
- 3384 Phospho-NMDAR1 (Ser896) Antibody
- 3385 Phospho-NMDAR1 (Ser897) Antibody
- 4781 Phospho-PKA C (Thr197) Antibody
- 4782 PKA C-α Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
