Cell Signaling Technology

Product Pathways - Neuroscience

NMDAR2B Antibody #4207

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Endogenous 190 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

NMDAR2B Antibody detects endogenous levels of total NMDAR2B protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human NMDAR2B. Antibodies are purified by peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from mouse and rat brain using NMDAR2B Antibody.

Background

N-methyl-D-aspartate receptor (NMDAR) forms a heterodimer of at least one NR1 and one NR2A-D subunit. Multiple receptor isoforms with distinct brain distributions and functional properties arise by selective splicing of the NR1 transcripts and differential expression of the NR2 subunits. NR1 subunits bind the co-agonist glycine and NR2 subunits bind the neurotransmitter glutamate. Activation of the NMDA receptor or opening of the ion channel allows flow of Na+ and Ca2+ ions into the cell, and K+ out of the cell (1). Each subunit has a cytoplasmic domain that can be directly modified by the protein kinase/phosphatase (2). PKC can phosphorylate the NR1 subunit (NMDAR1) of the receptor at Ser890/Ser896, and PKA can phosphorylate NR1 at Ser897 (3). The phosphorylation of NR1 by PKC decreases its affinity for calmodulin, thus preventing the inhibitory effect of calmodulin on NMDAR (4). The phosphorylation of NR1 by PKA probably counteracts the inhibitory effect of calcineurin on the receptor (5). NMDAR mediates long-term potentiation and slow postsynaptic excitation, which play central roles in learning, neurodevelopment, and neuroplasticity (6).

EphrinB2 binding to the receptor EphB leads to the activation of Src family tyrosine kinases, which phosphorylate NMDAR2B at Tyr1252, Tyr1336 and Tyr1472. In turn, phosphorylated NMDAR2B enhances the ability of the functional NMDA receptor to regulate Ca2+ influx in response to glutamate (7).

  1. Liu, X.B. et al. (2004) J. Neurosci. 24, 8885-8895.
  2. Westphal, R.S. et al. (1999) Science 285, 93-96.
  3. Tingley, W.G. et al. (1997) J. Biol. Chem. 272, 5157-5166.
  4. Hisatsune, C. et al. (1997) J. Biol. Chem. 272, 20805-20810.
  5. Raman, I.M. et al. (1996) Neuron 16, 415-421.
  6. Makhinson, M. et al. (1999) J. Neurosci. 19, 2500-2510.
  7. Takasu, M.A. et al. (2002) Science 295, 491-495.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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