Cell Signaling Technology

Product Pathways - Neuroscience

Phospho-NMDAR2B (Tyr1070) Antibody #4209

Applications Reactivity Sensitivity MW (kDa) Source
W R (H) (M) Endogenous 200 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Phospho-NMDAR2B (Tyr1070) Antibody detects endogenous levels of NMDAR2B only when phosphorylated at Tyr1070.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr1070 of NMDAR2B. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from rat brain, either sham-operated, 15 min ischemia followed by 4 h reperfusion or 15 min ischemia only, using Phospho-NMDAR2B (Tyr1070) Antibody (upper) or NMDAR2B #4207 Antibody (lower).

Background

N-methyl-D-aspartate receptor (NMDAR) forms a heterodimer of at least one NR1 and one NR2A-D subunit. Multiple receptor isoforms with distinct brain distributions and functional properties arise by selective splicing of the NR1 transcripts and differential expression of the NR2 subunits. NR1 subunits bind the co-agonist glycine and NR2 subunits bind the neurotransmitter glutamate. Activation of the NMDA receptor or opening of the ion channel allows flow of Na+ and Ca2+ ions into the cell, and K+ out of the cell (1). Each subunit has a cytoplasmic domain that can be directly modified by the protein kinase/phosphatase (2). PKC can phosphorylate the NR1 subunit (NMDAR1) of the receptor at Ser890/Ser896, and PKA can phosphorylate NR1 at Ser897 (3). The phosphorylation of NR1 by PKC decreases its affinity for calmodulin, thus preventing the inhibitory effect of calmodulin on NMDAR (4). The phosphorylation of NR1 by PKA probably counteracts the inhibitory effect of calcineurin on the receptor (5). NMDAR mediates long-term potentiation and slow postsynaptic excitation, which play central roles in learning, neurodevelopment, and neuroplasticity (6).

Phosphorylation of NMDAR2B at Tyr1070 was identified at Cell Signaling Technology (CST) using PhosphoScan®, CST's MS/MS platform for phosphorylation site discovery. Phosphorylation of NMDAR2B at Tyr1070 was observed in extracts isolated from ischemic rat brain. For additional information please visit PhosphoSitePlus®, CST's modification site knowledgebase, at www.phosphosite.org.

  1. Liu, X.B. et al. (2004) J. Neurosci. 24, 8885-8895.
  2. Westphal, R.S. et al. (1999) Science 285, 93-96.
  3. Tingley, W.G. et al. (1997) J. Biol. Chem. 272, 5157-5166.
  4. Hisatsune, C. et al. (1997) J. Biol. Chem. 272, 20805-20810.
  5. Raman, I.M. et al. (1996) Neuron 16, 415-421.
  6. Makhinson, M. et al. (1999) J. Neurosci. 19, 2500-2510.

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