Product Pathways - Neuroscience
Parkin (Prk8) Mouse mAb #4211
PhosphoSitePlus® protein, site, and accession data: PARK2
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP | H M R | Endogenous | 50 | Mouse IgG2b |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
Parkin (Prk8) Mouse mAb detects endogenous levels of total parkin protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with human recombinant parkin and the epitope maps to the carboxy terminus of the protein.
Western Blotting
Western blot analysis of extracts from PC12 cells, fetal rat brain and mouse brain, using Parkin (Prk8) Mouse mAb.
Background
Parkin is a protein of 465 amino acids with an amino-terminal ubiquitin domain and a carboxy-terminal RING-box (1). In the case of autosomal recessive juvenile Parkinsonism (AR-JP), deletions have been found in the gene on chromosome 6 encoding the protein Parkin (2).
- Tanner, C.M. and Goldman, S.M. (1996) Neurol. Clin. 14, 317-335.
- Polymeropoulous, M.H. et al. (1997) Science 276, 2045-2047.
Application References
- Pawlyk, A.C. et al. (2003) J. Biol. Chem. 278, 48120-8. Applications: IC-IF IP Western Blotting
- von Coelln, R. et al. (2006) J. Neurosci. 26, 3685-96. Applications: Western Blotting
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
