Cell Signaling Technology

Product Pathways - Protein Stability

USP28 Antibody #4217

Applications Reactivity Sensitivity MW (kDa) Source
W M (H) Transfected Only 135 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

USP28 Antibody detects transfected levels of USP28 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala11 of human USP28. Antibodies are purified by peptide affinity chromatography.

Western blot analysis of extracts from COS cells, untransfected or transfected with mouse USP28, using USP28 Antibody.

Background

Ubiquitinating enzymes (UBEs) catalyze protein ubiquitination, a reversible process countered by deubiquitinating enzymes (DUB) action (1,2). There are five DUB subfamilies including the USP, UCH, OTU, MJD and JAMM enzymes. USP28 is an important member of the USP subfamily (3). USP28 can bind to and deubiquinate several target proteins in DNA-damage pathway, resulting in their stability, including p53BP1 and Chk2 (4). USP28 also plays an important role in Myc related signaling by binding through FBW7α to Myc . It catalyzes the deubiquitinylation of Myc, thereby promoting its stabilization and contributing to tumor-cell growth (5).

  1. Nijman, S.M. et al. (2005) Cell 123, 773-86.
  2. Nalepa, G. et al. (2006) Nat Rev Drug Discov 5, 596-613.
  3. Valero, R. et al. (2001) Genome Biol 2, RESEARCH0043.
  4. Zhang, D. et al. (2006) Cell 126, 529-42.
  5. Popov, N. et al. (2007) Nat Cell Biol 9, 765-74.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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