Product Pathways - MAPK Signaling
Dexras1 Antibody #4229
PhosphoSitePlus® protein, site, and accession data: RASD1
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H | Endogenous | 30 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 4229:
- Western Blotting
Specificity / Sensitivity
Dexras1 Antibody detects endogenous levels of total Dexras1 protein. The antibody also cross-reacts with a 60 kDa protein of unknown origin.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Dexras1 protein. Antibodies were purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from HeLa and Hep G2 cells using Dexras1 Antibody.
Background
Dexras1 (Ras dexamethasone induced 1) belongs to the Ras superfamily of GTPases and was initially identified as a dexamethasone inducible gene (1,2). Dexras1 reportedly regulates several distinct signal transduction pathways, including MAPK signaling, NMDA receptor-nitric oxide-mediated signaling, and pathways involving adenylyl cyclases (3-5). Dexras1 can directly modulate FE65-APP-mediated transcription and regulate the photic sensitivity of the mammalian circadian clock (6,7).
- Kemppainen, R.J. and Behrend, E.N. (1998) J Biol Chem 273, 3129-31.
- Tu, Y. and Wu, C. (1999) Biochim Biophys Acta 1489, 452-6.
- Fang, M. et al. (2000) Neuron 28, 183-93.
- Graham, T.E. et al. (2004) Biochem Biophys Res Commun 316, 307-12.
- Nguyen, C.H. and Watts, V.J. (2005) Biochem Biophys Res Commun 332, 913-20.
- Cheng, H.Y. et al. (2006) J Neurosci 26, 12984-95.
- Lau, K.F. et al. (2008) J Biol Chem 283, 34728-37.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
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- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
For Research Use Only. Not For Use In Diagnostic Procedures.
