Product Pathways - Neuroscience
nNOS (C7D7) Rabbit mAb #4231
PhosphoSitePlus® protein, site, and accession data: nNOS
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP IHC-P IF-F | H M R | Endogenous | 160 | Rabbit IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IHC-P=Immunohistochemistry (Paraffin)
IF-F=Immunofluorescence (Frozen)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
nNOS (C7D7) Rabbit mAb detects endogenous levels of total nNOS protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser280 of human nNOS.
Western Blotting
Western blot analysis of extracts from mouse and rat brain using nNOS (C7D7) Rabbit mAb.
Western Blotting
Western blot analysis of extracts from wild-type (+/+) and nNOS null (-/-) mouse brain using nNOS (C7D7) Rabbit mAb (upper) and PSD95 Antibody #2507 (lower).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded wild-type (+/+, left) or nNOS null (-/-, right) mouse brain, using nNOS (C7D7) Rabbit mAb.
IF-F
Confocal immunofluorescent analysis of wild-type (+/+, upper) and nNOS null mouse brain (-/-, lower) using nNOS (C7D7) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Background
Nitric Oxide Synthase (NOS) catalyses the formation of nitric oxide (NO) and citruline from L-arginine, oxygen and cofactors. Three family members have been characterized: neuronal NOS (nNOS), which is found primarily in neuronal tissue; inducible NOS (iNOS), which is induced by interferon gamma and lipopolysaccharides in the kidney and cardiovascular system; and endothelial NOS (eNOS), which is expressed in blood vessels (1). NO is a messenger molecule with diverse functions throughout the body including the maintenance of vascular integrity, homeostasis, synaptic plasticity, long-term potentiation, learning, and memory (2,3).
- Tsutsui, M. (2004) J Atheroscler Thromb 11, 41-48.
- Son, H. et al. (1996) Cell 87, 1015-10123.
- Hawkins, R.D. (1996) Neuron 16, 465-467.
Application References
- Wu, M. et al. (2009) PLoS One 4, e6430. Applications: Western Blotting
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- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 9997 Tris Buffered Saline with Tween 20 (TBST-10X)
- 9998 BSA
- 9999 Nonfat Dry Milk
- 8114 SignalStain® Boost IHC Detection Reagent (HRP, Rabbit)
- 5425 Normal Goat Serum
- 8112 SignalStain® Antibody Diluent
Rabbit monoclonal antibody is produced under license (granting certain rights including those under U. S. Patent No. 5,675,063) from Epitomics, Inc.
For Research Use Only. Not For Use In Diagnostic Procedures.
