Cell Signaling Technology

Product Pathways - Neuroscience

nNOS Antibody #4234

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Endogenous 160 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

nNOS Antibody detects endogenous levels of total nNOS protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human nNOS. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from mouse and rat brain, using nNOS Antibody.

Background

Nitric Oxide Synthase (NOS) catalyses the formation of nitric oxide (NO) and citruline from L-arginine, oxygen and cofactors. Three family members have been characterized: neuronal NOS (nNOS), which is found primarily in neuronal tissue; inducible NOS (iNOS), which is induced by interferon gamma and lipopolysaccharides in the kidney and cardiovascular system; and endothelial NOS (eNOS), which is expressed in blood vessels (1). NO is a messenger molecule with diverse functions throughout the body including the maintenance of vascular integrity, homeostasis, synaptic plasticity, long-term potentiation, learning, and memory (2,3).

nNOS binds selectively to the second PDZ domain of PSD-95, and the enzymatic activity of nNOS is either downregulated by phosphorylation at Ser847 by CaM-KII or stimulated by calcium influxes governed by the NMDA receptor channels (4-5).

  1. Tsutsui, M. (2004) J Atheroscler Thromb 11, 41-48.
  2. Son, H. et al. (1996) Cell 87, 1015-10123.
  3. Hawkins, R.D. (1996) Neuron 16, 465-467.
  4. Watanabe, Y. et al. (2003) Biochem J 372, 465-71.
  5. Sears, C.E. et al. (2004) Philos Trans R Soc Lond B Biol Sci 359, 1021-44.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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