Product Pathways - Neuroscience
NG2 Antibody #4235
|W||H R (M)||Endogenous||250||Rabbit|
Reactivity Key: H=Human M=Mouse R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
NG2 Antibody detects endogenous levels of total NG2 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide surrounding His2260 of human NG2. Antibodies are purified by peptide affinity chromatography.
The chondroitin sulfate proteoglycan NG2 is a type I membrane protein expressed by subpopulations of glia including oligodendroglial precursor cells and a variety of tumor cells. Normal precursor cells and malignant tumor cells migrate and proliferate, but there is evidence that cells may not be able to engage in both activities at the same time. However, NG2 is involved in promoting both proliferation and motility (1). The extracellular domain of NG2 sequesters growth factors and binds to both growth factor receptors and extracellular matrix ligands such as fibronectin, collagens and laminin. The cytoplasmic domain is involved in activating Rac, Cdc42 and p130 Cas (2). PKCα phosphorylates NG2 at Thr2256, triggering the redistribution of NG2 from apical microprocesses to lamellipodia accompanied by enhanced cell motility (3). ERK phosphorylates NG2 at Thr2314, stimulating cell proliferation (4).
- Karram, K. et al. (2005) J Anat 207, 735-44.
- Eisenmann, K.M. et al. (1999) Nat Cell Biol 1, 507-13.
- Makagiansar, I.T. et al. (2004) J Biol Chem 279, 55262-70.
- Makagiansar, I.T. et al. (2007) J Cell Biol 178, 155-65.
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For Research Use Only. Not For Use In Diagnostic Procedures.