Cell Signaling Technology

Product Pathways - Neuroscience

NG2 Antibody #4235

Applications Reactivity Sensitivity MW (kDa) Source
W H R (M) Endogenous 250 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

NG2 Antibody detects endogenous levels of total NG2 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide surrounding His2260 of human NG2. Antibodies are purified by peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from MDA-MB-468 and RN33B cells using NG2 Antibody.

Background

The chondroitin sulfate proteoglycan NG2 is a type I membrane protein expressed by subpopulations of glia including oligodendroglial precursor cells and a variety of tumor cells. Normal precursor cells and malignant tumor cells migrate and proliferate, but there is evidence that cells may not be able to engage in both activities at the same time. However, NG2 is involved in promoting both proliferation and motility (1). The extracellular domain of NG2 sequesters growth factors and binds to both growth factor receptors and extracellular matrix ligands such as fibronectin, collagens and laminin. The cytoplasmic domain is involved in activating Rac, Cdc42 and p130 Cas (2). PKCα phosphorylates NG2 at Thr2256, triggering the redistribution of NG2 from apical microprocesses to lamellipodia accompanied by enhanced cell motility (3). ERK phosphorylates NG2 at Thr2314, stimulating cell proliferation (4).

  1. Karram, K. et al. (2005) J Anat 207, 735-44.
  2. Eisenmann, K.M. et al. (1999) Nat Cell Biol 1, 507-13.
  3. Makagiansar, I.T. et al. (2004) J Biol Chem 279, 55262-70.
  4. Makagiansar, I.T. et al. (2007) J Cell Biol 178, 155-65.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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