Cell Signaling Technology

Product Pathways - Translational Control

TFEB Antibody #4240

Applications Reactivity Sensitivity MW (kDa) Source
W IP H Endogenous 65-70 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

TFEB Antibody recognizes endogenous levels of total human TFEB protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly412 of human TFEB protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using TFEB Antibody (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).

IP

IP

Immunoprecipitation of TFEB from COLO 205 cells using TFEB Antibody (lane 2) or Normal Rabbit IgG #2729 (lane 3). Lane 1 is 10% input.

Specificity

Specificity

Western blot analysis of extracts from Raji cells, untreated (-) or Torin1-treated (250 nM, 1 hr; +), using TFEB Antibody.


Background

Transcription factor EB (TFEB) is a member of the Myc-related, bHLH leucine-zipper family of transcription factors that drives the expression of a network of genes known as the Coordinated Lysosomal Expression and Regulation (CLEAR) network (1,2). TFEB specifically recognizes and binds regulatory sequences within the CLEAR box (GTCACGTGAC) of lysosomal and autophagy genes, resulting in the up-regulated expression of genes involved in lysosome biogenesis and function, and regulation of autophagy (1,2). TFEB is activated in response to nutrient deprivation, stimulating translocation to the nucleus where it forms homo- or heterooligomers with other members of the microphthalmia transcription factor (MiTF) subfamily and resulting in up-regulation of autophagosomes and lysosomes (3-5). Recently, it has been shown that TFEB is a component of mammalian target of rapamycin (mTOR) complex 1 (mTORC1), which regulates the phosphorylation and nuclear translocation of TFEB in response to cellular starvation and stress (6-9). During normal growth conditions, TFEB is phosphorylated at Ser211 in an mTORC1-dependent manner. Phosphorylation promotes association of TFEB with 14-3-3 family proteins and retention in the cytosol. Inhibition of mTORC1 results in a loss of TFEB phosphorylation, dissociation of the TFEB/14-3-3 complex, and rapid transport of TFEB to the nucleus where it increases transcription of CLEAR and autophagy genes (10). TFEB has also been shown to be activated in a nutrient-dependent manner by p42 MAP kinase (Erk2). TFEB is phosphorylated at Ser142 by Erk2 in response to nutrient deprivation, resulting in nuclear localization and activation, and indicating that pathways other than mTOR contribute to nutrient sensing via TFEB (3).

  1. Sardiello, M. et al. (2009) Science 325, 473-7.
  2. Sardiello, M. and Ballabio, A. (2009) Cell Cycle 8, 4021-2.
  3. Settembre, C. et al. (2011) Science 332, 1429-33.
  4. David, R. (2011) Nat Rev Mol Cell Biol 12, 404.
  5. Cuervo, A.M. (2011) Science 332, 1392-3.
  6. Peña-Llopis, S. et al. (2011) EMBO J 30, 3242-58.
  7. Settembre, C. and Ballabio, A. (2011) Autophagy 7, 1379-81.
  8. Peña-Llopis, S. and Brugarolas, J. (2011) Cell Cycle 10, 3987-8.
  9. Settembre, C. et al. (2012) EMBO J 31, 1095-108.
  10. Martina, J.A. et al. (2012) Autophagy 8, .

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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