Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

BCL6 Antibody #4242

Applications Reactivity Sensitivity MW (kDa) Source
W H M (R) Endogenous 87 to 98 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

The BCL6 Antibody detects endogenous levels of BCL6.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy-terminal sequence of human BCL6. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of Ramos and A20 cell extracts using BCL6 Antibody.

Background

Chromosomal translocations result in misregulation of the proto-oncogene BCL6 in patients with B cell-derived non-Hodgkins lymphoma (1). The BCL6 gene is selectively expressed in mature B cells and encodes a nuclear phosphoprotein that belongs to the BTB/POZ zinc finger family of transcription factors (2,3). BCL6 protein can bind to target DNA sequences of Stat6 and, analogous to Stat6, modulate the expression of interleukin-4-induced genes (4). Furthermore, BCL6 restrains p53-dependent senescence, making BCL6-active tumors functionally p53-negative (5). The mitogen-activated protein kinases, Erk1 and Erk2, but not JNK, phosphorylate BCL6 at multiple sites. Phosphorylation of BCL6 at Ser333 and Ser343 results in degradation of BCL6 by the ubiquitin/proteasome pathway in B cells (6,7). In addition, BCL6 is acetylated and its transcriptional repressor function is inhibited by the transcriptional co-activator p300 (8).

  1. Ye, B. H. et al. (1993) Science 262, 747-750.
  2. Onizuka, T. et al. (1995) Blood 86, 28-37.
  3. Zollman, S. et al. (1994) Proc. Natl. Acad. Sci. USA 91, 10717-10721.
  4. Harris, M. B. et al. (1999) Mol. Cell. Biol. 19, 7264-7275.
  5. Shvarts, A. et al. (2002) Genes Dev. 16, 681-686.
  6. Moriyama, M. et al. (1997) Oncogene 14, 2465-2474.
  7. Niu, H. et al. (1998) Genes Dev. 12, 1953-1961.
  8. Bereshchenko, O. R. et al. (2002) Nat. Genet. 32, 606-613.

Application References

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Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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