Product Pathways - PI3K / Akt Signaling
PI3 Kinase p110α (C73F8) Rabbit mAb #4249
|4249S||100 µl (10 western blots)||---||In Stock||---|
|4249P||40 µl (4 western blots)||---||In Stock||---|
|4249||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat, Bovine||Endogenous||110||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin)
Specificity / Sensitivity
PI3 Kinase p110α (C73F8) Rabbit mAb detects endogenous levels of total PI3K p110α protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide surrounding Asp520 of the sequence of human PI3K p110α.
Western blot analysis of extracts from HeLa cells and neonatal mouse brain using PI3 Kinase p110α (C73F8) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using PI3 Kinase p110α (C73F8) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using PI3 Kinase p110α (C73F8) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).
Phosphoinositide 3-kinase (PI3K) catalyzes the production of phosphatidylinositol-3,4,5-triphosphate by phosphorylating phosphatidylinositol (PI), phosphatidylinositol-4-phosphate (PIP), and phosphatidylinositol-4,5-bisphosphate (PIP2). Growth factors and hormones trigger this phosphorylation event, which in turn coordinates cell growth, cell cycle entry, cell migration, and cell survival (1). PTEN reverses this process, and research studies have shown that the PI3K signaling pathway is constitutively activated in human cancers that have loss of function of PTEN (2). PI3Ks are composed of a catalytic subunit (p110) and a regulatory subunit. Various isoforms of the catalytic subunit (p110α, p110β, p110γ, and p110δ) have been isolated, and the regulatory subunits that associate with p110α, p110β, and p110δ are p85α and p85β (3). In contrast, p110γ associates with a p101 regulatory subunit that is unrelated to p85. Furthermore, p110γ is activated by βγ subunits of heterotrimeric G proteins (4).
- Popkie, A.P. et al. (2010) J Biol Chem 285, 41337-47. Applications: Western Blotting.
- Yoshioka, K. et al. (2012) Nat Med 18, 1560-9. Applications: Western Blotting.
- Biswas, K. et al. (2013) J Biol Chem 288, 2325-39. Applications: Western Blotting.
- Ramadani, F. et al. (2010) Sci Signal 3, ra60. Applications: Western Blotting.
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For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
This antibody is developed, validated, and produced by CST using in part technology under license (granting certain rights including those under U.S. Patent No. 5,675,063) from Epitomics, Inc.