Product Pathways - Akt Signaling
PI3 Kinase p110α Antibody #4255
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP | H M R (B) | Endogenous | 110 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
M=Mouse
R=Rat
B=Bovine
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
PI3 Kinase p110α Antibody detects endogenous levels of total PI3K p110α protein.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) derived from the human sequence of PI3K p110α. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western Blot analysis of Jurkat cell extracts treated with LY294002 (PI3 Kinase Inhibitor) #9901 or Calyculin A #9902 using PI3 Kinase p110α Antibody.
Background
Phosphoinositide 3-kinase (PI3K) catalyzes the production of phosphatidylinositol-3,4,5-triphosphate by phosphorylating phosphatidylinositol (PI), phosphatidylinositol-4-phosphate (PIP) and phosphatidylinositol-4,5-bisphosphate (PIP2). Growth factors and hormones trigger this phosphorylation event, which in turn coordinates cell growth, cell cycle entry, cell migration and cell survival (1). PTEN reverses this process, and the PI3K signaling pathway is constitutively activated in human cancers that have loss of function of PTEN (2). PI3Ks are composed of a catalytic subunit (p110) and a regulatory subunit. Various isoforms of the catalytic subunit (p110α, p110β, p110γ and p110δ) have been isolated, and the regulatory subunits that associate with p110α, p110β and p110δ are p85α and p85β (3). In contrast, p110γ associates with a p101 regulatory subunit that is unrelated to p85. Furthermore, p110 γ is activated by βγ subunits of heterotrimeric G proteins (4).
- Cantley, L.C. (2002) Science 296, 1655-7.
- Simpson, L. and Parsons, R. (2001) Exp Cell Res 264, 29-41.
- Neri, L.M. et al. (2002) Biochim Biophys Acta 1584, 73-80.
- Stoyanov, B. et al. (1995) Science 269, 690-3.
Application References
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Companion Products
- 9901 LY294002 (PI3 Kinase Inhibitor)
- 9916 Phospho-Akt Pathway Sampler Kit
- 9551 Phospho-PTEN (Ser380) Antibody
- 9552 PTEN Antibody
- 9559 PTEN (138G6) Rabbit mAb
- 9556 PTEN (26H9) Mouse mAb
- 4252 PI3 Kinase p110γ Antibody
- 4292 PI3 Kinase p85 Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7727 Biotinylated Protein Ladder Detection Pack
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 4228 Phospho-PI3K p85 (Tyr458)/p55 (Tyr199) Antibody
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
