Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

MLH1 (D38G9) Rabbit mAb #4256

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H Mk Endogenous 84 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

MLH1 (D38G9) Rabbit mAb detects endogenous levels of total MLH1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human MLH1 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using MLH1 (D38G9) Rabbit mAb.

IP

IP

Immunoprecipitation of MLH1 from HeLa cells using MLH1 (D38G9) Rabbit mAb. Western blot was performed using the same antibody. IP () is 5% input.

Background

Mismatch repair (MMR), a conserved process that involves correcting errors made during DNA synthesis, is crucial to the maintenance of genomic integrity. MLH1 is the human homologue of the E. coli MMR gene mutL. MMR requires recognition of a base mismatch or insertion/deletion loop by a MutS homolog followed by recruitment of a MutL heterodimeric complex consisting of MLH1 and PMS1 (MutL-α), PMS2 (MutL-β) or MLH3 (MutL-γ). Other factors required for MMR in eukaryotes are EXO1, PCNA, RFC, RPA, DNA polymerases and DNA ligase (reviewed in 1). Inactivation of the MLH1 gene causes genome instability and predisposition to cancer (2-5). The MLH1 gene is frequently mutated in hereditary nonpolyposis colon cancer (HNPCC) (6). MLH1 also plays a role in meiotic recombination (7).

  1. Modrich, P. (2006) J Biol Chem 281, 30305-9.
  2. Seng, T.J. et al. (2008) Br J Cancer 99, 375-82.
  3. Harley, I. et al. (2008) Gynecol Oncol 109, 384-7.
  4. Mao, G. et al. (2008) J Biol Chem 283, 3211-6.
  5. Hubner, R.A. and Houlston, R.S. (2007) J Natl Cancer Inst 99, 1490; author reply 1490-1.
  6. Vasen, H.F. (2005) Fam Cancer 4, 219-25.
  7. Argueso, J.L. et al. (2003) Mol Cell Biol 23, 873-86.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

Products