Product Pathways - Akt Signaling
PI3 Kinase p85 (19H8) Rabbit mAb #4257
| Applications | Reactivity | MW (kDa) | Source | Isotype |
|---|---|---|---|---|
| W IP | H M R | 85 | Rabbit | IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
PI3 Kinase p85 (19H8) Rabbit mAb detects endogenous levels of total PI3K p85 protein.
Source / Purification
Monoclonal antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) derived from the sequence of human PI3K p85.
Western Blotting
Western blot analysis of extracts from Jurkat and NIH/3T3 cells, using PI3 Kinase p85 (19H8) Rabbit mAb.
Background
Phosphoinositide 3-kinase (PI3K) catalyzes the production of phosphatidylinositol-3,4,5-triphosphate by phosphorylating phosphatidylinositol (PI), phosphatidylinositol-4-phosphate (PIP) and phosphatidylinositol-4,5-bisphosphate (PIP2). Growth factors and hormones trigger this phosphorylation event, which in turn coordinates cell growth, cell cycle entry, cell migration and cell survival (1). PTEN reverses this process, and the PI3K signaling pathway is constitutively activated in human cancers that have loss of function of PTEN (2). PI3Ks are composed of a catalytic subunit (p110) and a regulatory subunit. Various isoforms of the catalytic subunit (p110α, p110β, p110γ and p110δ) have been isolated, and the regulatory subunits that associate with p110α, p110β and p110δ are p85α and p85β (3). In contrast, p110γ associates with a p101 regulatory subunit that is unrelated to p85. Furthermore, p110 γ is activated by βγ subunits of heterotrimeric G proteins (4).
- Cantley, L.C. (2002) Science 296, 1655-7.
- Simpson, L. and Parsons, R. (2001) Exp Cell Res 264, 29-41.
- Neri, L.M. et al. (2002) Biochim Biophys Acta 1584, 73-80.
- Stoyanov, B. et al. (1995) Science 269, 690-3.
Application References
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- 4255 PI3 Kinase p110α Antibody
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.
