Product Pathways - MAPK Signaling
Phospho-SRF (Ser103) Antibody #4261
PhosphoSitePlus® protein, site, and accession data: SRF
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H M R | Endogenous | 67 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 4261:
- Western Blotting
Specificity / Sensitivity
Phospho-SRF (Ser103) Antibody detects endogenous levels of SRF only when phosphorylated at serine 103. This antibody cross-reacts with a non-specific protein of approximately 52 kDa.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around serine 103 of human SRF. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from NIH/3T3 and HeLa cells untreated or TPA-treated, using Phospho-SRF (Ser103) Antibody.
Background
The serum response factor (SRF) is a 67 kDa phospho-protein that, together with auxiliary factors, modulates transcription of immediate early genes containing serum response elements at their promoters (1,2). SRF contains several phosphorylation sites (3), but functional consequences of phosphorylation have not been identified unequivocally. Several growth factor- and calcium-regulated kinases, such as p90RSK and CaM kinase IV, can phosphorylate SRF at Ser103 (4,5), and Ser103 of SRF is also a nuclear target for MAPKAP kinase 2 (6).
- Treisman, R. (1995) EMBO J. 14, 4905-4913.
- Janknecht, R. et al. (1995) Carcinogenesis 16, 443-450.
- Marais, R. M. et al. (1992) EMBO J. 11, 97-105.
- Rivera, V. M. et al. (1993) Mol. Cell. Biol. 13, 6260-6273.
- Miranti, C. K. et al. (1995) Mol. Cell. Biol. 15, 3672-3684.
- Heidenreich, O. et al. (1999) JBC 274, 14434-14443.
Application References
- Xifró, X. et al. (2011) Mol Neurodegener 6, 74. Applications: Western Blotting
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For Research Use Only. Not For Use In Diagnostic Procedures.
