Product Pathways - Cytoskeletal Signaling
Rab10 Antibody #4262
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H M Mk (R) | Endogenous | 23 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by Western blot.
Protocols
- 4262:
- Western Blotting
Specificity / Sensitivity
Rab10 Antibody detects endogenous levels of total Rab10 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp114 of human Rab10 protein. Antibodies are purified by peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from various cell lines using Rab10 Antibody.
Background
Rab10 is a member of the Ras superfamily of small Rab GTPases (1) that interacts with Msst4, myosin V (Va, Vb and Vc), and GDI as it helps mediate sorting among cellular endosomes (2-4). Mutation analysis and GFP-fusion protein expression of Rab10 in MDCK cells determined that Rab10 plays a regulatory role in membrane protein transport between early endosome and basolateral compartments (5,6). Rab10 associates with the GLUT4 complex as a target for AS160 and is required for translocation of GLUT4 under insulin stimulation in adipocytes (7,8).
- Chen, Y.T. et al. (1993) Proc Natl Acad Sci USA 90, 6508-12.
- Burton, J.L. et al. (1994) EMBO J 13, 5547-58.
- Roland, J.T. et al. (2009) J Biol Chem 284, 1213-23.
- Chen, Y. et al. (2009) Biochem J 422, 229-35.
- Schuck, S. et al. (2007) Traffic 8, 47-60.
- Babbey, C.M. et al. (2006) Mol Biol Cell 17, 3156-75.
- Sano, H. et al. (2007) Cell Metab 5, 293-303.
- Larance, M. et al. (2005) J Biol Chem 280, 37803-13.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
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- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
