Cell Signaling Technology

Product Pathways - PI3K / Akt Signaling

PI3 Kinase Class III (D9A5) Rabbit mAb #4263

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H M R Mk Endogenous 100 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

PI3 Kinase Class III (D9A5) Rabbit mAb detects endogenous levels of total PI3K class III protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide surrounding Lys630 of human PI3 kinase class III.

Western Blotting

Western Blotting

Western blot analysis of extracts from mouse brain and C6 cells using PI3 Kinase Class III (D9A5) Rabbit mAb.

Background

Three distinct types of phosphoinositide 3-kinases (PI3K) have been characterized. Unlike other PI3Ks, PI3K class III catalyzes the phosphorylation of phosphatidylinositol at the D3 position, producing phosphatidylinositol-3-phosphate (PIP3) (1). PI3K class III is the mammalian homolog of Vps34, first identified in yeast. PI3K class III interacts with the regular subunit p150, the mammalian homolog of Vps15, which regulates cellular membrane association through myristoylation (2,3). PIP3 recruits several proteins with FYVE or PX domains to membranes regulating vesicular transport and protein sorting (4). Moreover, PI3K class III has been shown to regulate autophagy, trimeric G-protein signaling, and the mTOR nutrient-sensing pathway (5).

  1. Odorizzi, G. et al. (2000) Trends Biochem Sci 25, 229-35.
  2. Panaretou, C. et al. (1997) J Biol Chem 272, 2477-85.
  3. Kihara, A. et al. (2001) J Cell Biol 152, 519-30.
  4. Corvera, S. (2001) Traffic 2, 859-66.
  5. Yan, Y. and Backer, J.M. (2007) Biochem Soc Trans 35, 239-41.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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