Product Pathways - Neuroscience
STOP (175) Mouse mAb #4265
|W IF-F||H M R||Endogenous||120||Mouse IgG1|
Reactivity Key: H=Human M=Mouse R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
STOP (175) Mouse mAb detects endogenous levels of total N-STOP and O-STOP proteins.
Source / Purification
Monoclonal antibody is produced by immunizing animals with purified N-STOP from rat brain. The antigen is encoded by exon 4 (Galiano et al., 2004).
Western blot analysis of extracts from wild type (WT) and N-STOP knock-out mouse brain lysates, using STOP (175) Mouse mAb (upper) or Actin Antibody (lower). (Kindly provided by Dr. Robert Margolis, Sidney Kimmel Cancer Center, San Diego, California).
Confocal immunofluorescent analysis of rat cerebellum (left) and forebrain (right) using STOP (175) Mouse mAb (green) and MAP2 Antibody #4542 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Stable Tubule Only Polypeptide (STOP) is a microtubule-associated protein, and its microtubule-stabilizing activity is regulated by calmodulin (1-2). STOPs have several tissue- and developmental-specific isoforms that are encoded by a single gene. Neurons express N-STOP (exons 1-4) and E-STOP (exons 1-3), fibroblasts express F-STOP (exons 1-2), oligodendrocytes express O-STOP, and astrocytes A-STOP (3). STOPs are the major contributors stabilizing microtubules that resist depolymerization due to cold or depolymerizing drugs. STOP knock-out mice display impaired synaptic plasticity associated with severe behavioral disorders in contrast to the anticipated neuronal development and brain anatomy defects (4).
- Bosc, C. et al. (1996) Proc Natl Acad Sci USA 93, 2125-30.
- Bosc, C. et al. (2001) J Biol Chem 276, 30904-13.
- Galiano, M.R. et al. (2004) J Neurosci Res 78, 329-37.
- Andrieux, A. et al. (2002) Genes Dev 16, 2350-64.
- Bosc, C. et al. (1996) Proc Natl Acad Sci USA 93, 2125-30. Applications: IC-IF Western Blotting
- Galiano, M.R. et al. (2004) J Neurosci Res 78, 329-37. Applications: Western Blotting
- Baratier, J. et al. (2006) J Biol Chem 281, 19561-9. Applications: IF-IC (In Cells) Western Blotting
- Ochoa, C.D. et al. (2011) Am J Physiol Lung Cell Mol Physiol 300, L132-8. Applications: Western Blotting
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
- 2148 α/β-Tubulin Antibody
- 4970 β-Actin (13E5) Rabbit mAb
- 2835 Neurofilament-L (DA2) Mouse mAb
- 2836 Neurofilament-H (RMdO 20) Mouse mAb
- 2838 Neurofilament-M (RMO 14.9) Mouse mAb
- 4830 Calmodulin Antibody
- 7072 Phototope®-HRP Western Blot Detection System, Anti-mouse IgG, HRP-linked Antibody
- 7076 Anti-mouse IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
For Research Use Only. Not For Use In Diagnostic Procedures.