Cell Signaling Technology

Product Pathways - Neuroscience

STOP (175) Mouse mAb #4265

Applications Reactivity Sensitivity MW (kDa) Isotype
W IF-F H M R Endogenous 120 Mouse IgG1

Applications Key:  W=Western Blotting  IF-F=Immunofluorescence (Frozen)
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

STOP (175) Mouse mAb detects endogenous levels of total N-STOP and O-STOP proteins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with purified N-STOP from rat brain. The antigen is encoded by exon 4 (Galiano et al., 2004).

Western Blotting

Western Blotting

Western blot analysis of extracts from wild type (WT) and N-STOP knock-out mouse brain lysates, using STOP (175) Mouse mAb (upper) or Actin Antibody (lower). (Kindly provided by Dr. Robert Margolis, Sidney Kimmel Cancer Center, San Diego, California).

Western Blotting

Western Blotting

Western blot analysis of extracts from rat brain using STOP (175) Mouse mAb.

IF-F

IF-F

Confocal immunofluorescent analysis of rat cerebellum (left) and forebrain (right) using STOP (175) Mouse mAb (green) and MAP2 Antibody #4542 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).


Background

Stable Tubule Only Polypeptide (STOP) is a microtubule-associated protein, and its microtubule-stabilizing activity is regulated by calmodulin (1-2). STOPs have several tissue- and developmental-specific isoforms that are encoded by a single gene. Neurons express N-STOP (exons 1-4) and E-STOP (exons 1-3), fibroblasts express F-STOP (exons 1-2), oligodendrocytes express O-STOP, and astrocytes A-STOP (3). STOPs are the major contributors stabilizing microtubules that resist depolymerization due to cold or depolymerizing drugs. STOP knock-out mice display impaired synaptic plasticity associated with severe behavioral disorders in contrast to the anticipated neuronal development and brain anatomy defects (4).

  1. Bosc, C. et al. (1996) Proc Natl Acad Sci USA 93, 2125-30.
  2. Bosc, C. et al. (2001) J Biol Chem 276, 30904-13.
  3. Galiano, M.R. et al. (2004) J Neurosci Res 78, 329-37.
  4. Andrieux, A. et al. (2002) Genes Dev 16, 2350-64.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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