Product Pathways - Neuroscience
STOP (175) Mouse mAb #4265
|4265S||100 µl (10 western blots)||---||In Stock||---|
|4265||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat||Endogenous||120||Mouse IgG1|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IF-F=Immunofluorescence (Frozen)
Specificity / Sensitivity
STOP (175) Mouse mAb detects endogenous levels of total N-STOP and O-STOP proteins.
Source / Purification
Monoclonal antibody is produced by immunizing animals with purified N-STOP from rat brain. The antigen is encoded by exon 4 (Galiano et al., 2004).
Western blot analysis of extracts from wild type (WT) and N-STOP knock-out mouse brain lysates, using STOP (175) Mouse mAb (upper) or Actin Antibody (lower). (Kindly provided by Dr. Robert Margolis, Sidney Kimmel Cancer Center, San Diego, California).
Confocal immunofluorescent analysis of rat cerebellum (left) and forebrain (right) using STOP (175) Mouse mAb (green) and MAP2 Antibody #4542 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Stable Tubule Only Polypeptide (STOP) is a microtubule-associated protein, and its microtubule-stabilizing activity is regulated by calmodulin (1-2). STOPs have several tissue- and developmental-specific isoforms that are encoded by a single gene. Neurons express N-STOP (exons 1-4) and E-STOP (exons 1-3), fibroblasts express F-STOP (exons 1-2), oligodendrocytes express O-STOP, and astrocytes A-STOP (3). STOPs are the major contributors stabilizing microtubules that resist depolymerization due to cold or depolymerizing drugs. STOP knock-out mice display impaired synaptic plasticity associated with severe behavioral disorders in contrast to the anticipated neuronal development and brain anatomy defects (4).
- Bosc, C. et al. (1996) Proc Natl Acad Sci USA 93, 2125-30. Applications: Western Blotting, IC-IF.
- Galiano, M.R. et al. (2004) J Neurosci Res 78, 329-37. Applications: Western Blotting.
- Baratier, J. et al. (2006) J Biol Chem 281, 19561-9. Applications: Western Blotting, IF-IC (In Cells).
- Ochoa, C.D. et al. (2011) Am J Physiol Lung Cell Mol Physiol 300, L132-8. Applications: Western Blotting.
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