Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

Fer (5D2) Mouse mAb #4268

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H M Endogenous 95 Mouse IgG1

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Fer (5D2) Mouse mAb detects endogenous levels of total Fer protein. This antibody does not cross-react with other related proteins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a truncated recombinant Fer protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3, A549 and SK-MEL-5 cells using Fer (5D2) Mouse mAb.

Background

Fer is a nonreceptor protein-tyrosine kinase of the Fes/Fps family. Like many other cytoplasmic tyrosine kinases, Fer contains a long amino-terminal domain, a central SH2 domain, and a carboxy-terminal kinase domain. Its amino-terminal domain is responsible for protein oligomerization as well as interaction with cytoskeletal proteins. Fer is ubiquitously expressed in a wide variety of cell and tissue types, and is localized to both cytoplasm and nucleus (1). Tyrosine kinase activity of Fer can be stimulated by growth factors and cytokines (2,3). After activation, Fer can further activate various downstream signaling components including Stat3 (3). Fer plays an important role in regulation of cell movement, oncogenesis and inflammation (4).

  1. Hao, Q.L. et al. (1991) Mol Cell Biol 11, 1180-3.
  2. Kim, L. and Wong, T.W. (1995) Mol Cell Biol 15, 4553-61.
  3. Zoubeidi, A. et al. (2009) Mol Cancer Res 7, 142-55.
  4. Greer, P. (2002) Nat Rev Mol Cell Biol 3, 278-89.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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