Product Pathways - Tyrosine Kinase / Adaptors
Ron (1B5) Mouse mAb #4269
Reactivity Key: H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
Ron (1B5) Mouse mAb detects endogenous levels of the 170 kDa full-length Ron protein. This antibody does not cross-react with other family members.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a recombinant Trx-Ron fusion protein containing amino acids 210-320 of human Ron expressed in E. coli.
Ron is a member of the Met protooncogene family of receptor tyrosine kinases, which also includes Stk, c-Met, and c-Sea. The functional Ron is a heterodimer composed of a 40 kDa α chain and a 150 kDa β chain. Ron is initially synthesized in the cells as a single-chain, pro-Ron precursor that is cleaved into the two active chains. The α chain is completely extracellular, whereas the β chain traverses the cell membrane and contains the intracellular tyrosine kinase and regulatory elements (1,2). Ron mediates multiple signaling cascades that involve cell motility, adhesion, proliferation, and apoptosis. The signaling pathways activated downstream of Ron include the ras/mitogen-activated protein kinase (MAPK), phosphatidyl inositol-3 kinase (PI3K)/Akt, and focal adhesion kinase (FAK) pathways. Ron activation can also significantly increase c-Src activity, a signaling intermediate involved in cell cycle progression, motility, angiogenesis and survival (3,4). The function of Ron has been shown to be important for embryological development as well as implicated in the progression and metastasis of tumors (5).
- Ronsin, C. et al. (1993) Oncogene 8, 1195-1202.
- Gaudino, G. et al. (1994) EMBO J. 13, 3524-3532.
- Wang, M.H. et al. (1996) Oncogene 13, 2167-2175.
- Danilkovitch-Miagkova, A. (2003) Curr. Cancer Drug Targets 3, 31-40.
- Leonard, E.J. (1997) Ciba Found. Symp. 212, 183-191; discussion 192-197.
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